Anti-FZR1, (N-terminal) antibody produced in rabbit
SIGMA/SAB2105778 - affinity isolated antibody
Synonym: Anti-CDC20C; Anti-CDH1; Anti-FZR; Anti-FZR2; Anti-HCDH
Product Type: Chemical
Immunoblotting Anti-FZR1 (N-Terminal): Cat. No. SAB2105778: FZR1 in 721_B cell lysates with FZR1 antibody at 1.0 μg/mL.
Immunoblotting FZR1: Cat. No. SAB2105778: Western blot analysis of FZR1 in Human Fetal Brain cell lysates with FZR1 antibody at 1.0 μg/mL.
Immunoblotting FZR1: Cat. No. SAB2105778: Western blot analysis of FZR1 in Human Fetal Heart cell lysates with FZR1 antibody at 1.0 μg/mL.
Immunoblotting FZR1: Cat. No. SAB2105778: Western blot analysis of FZR1 in Human Fetal Lung cell lysates with FZR1 antibody at 1.0 μg/mL.
Immunoblotting FZR1: Cat. No. SAB2105778: Western blot analysis of FZR1 in Human Adult Placenta cell lysates with FZR1 antibody at 1.0 μg/mL.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 55 kDa |
| NCBI accession no. | NM_016263   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | bovine, dog, human, horse, rat, mouse, guinea pig |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: suitable |
| UniProt accession no. | Q9UM11 |
| Biochem/physiol Actions: | FZR1 is a key regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C), which confers substrate specificity upon the complex. FZR1 associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | Synthetic peptide directed towards the N terminal region of human FZR1 |
| Other Notes: | Synthetic peptide located within the following region: SSPVSSPSKHGDRFIPSRAGANWSV |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |