Anti-POLI, (N-terminal) antibody produced in rabbit
SIGMA/SAB2105830 - affinity isolated antibody
Synonym: Anti-RAD30B; Anti-RAD3OB
Product Type: Chemical
Immunoblotting Anti-POLI (N-Terminal): Cat. No. SAB2105830: POLI in Fetal Lung cell lysates with POLI antibody at 1.0 μg/mL.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 83 kDa |
| NCBI accession no. | NM_007195   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | bovine, rabbit, rat, mouse, guinea pig, dog, horse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: suitable |
| UniProt accession no. | Q9UNA4 |
| Biochem/physiol Actions: | POLI is an error-prone DNA polymerase specifically involved in DNA repair.POLI plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls.POLI favors Hoogsteen base-pairing in the active site.POLI inserts the correct base with high-fidelity opposite an adenosine template.POLI exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine.POLI may play a role in hypermutation of immunogobulin genes.POLI forms a Schiff base with 5′-deoxyribose phosphate at abasic sites, but may not have lyase activity. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | Synthetic peptide directed towards the N terminal region of human POLI |
| Other Notes: | Synthetic peptide located within the following region: PVVERLGFDENFVDLTEMVEKRLQQ |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |