Anti-DPF3 antibody produced in rabbit
SIGMA/SAB2106452 - affinity isolated antibody
Product Type: Chemical
Immunohistochemistry DPF3 Antibody: Cat. No. SAB2106452: Immunohistochemistry analysis of DPF3 in heart with DPF3 Antibody 1.0 μg/mL. Observed Staining: Nuclear (not in cardiomyocytes but in fibrocytes in endomysium
Immunoblotting Anti-DPF3: Cat. No. SAB2106452: Western blot analysis of Dpf3 in Mouse Spleen with Dpf3 (D4, zinc and double PHD fingers, family 3) antibody against the N terminal of Dpf3 (50 μg) antibody at 1.0 μg/mL.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 40 kDa |
| NCBI accession no. | NM_058212   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, horse, human, rabbit, guinea pig, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| western blot: suitable | |
| UniProt accession no. | P58269-3 |
| Biochem/physiol Actions: | Dpf3 is a muscle-specific component of the BAF complex, a multiprotein complex involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Dpf3 specifically binds acetylated lysines on histone 3 and 4 (H3K14ac, H3K9ac, H4K5ac, H4K8ac, H4K12ac, H4K16ac). In the complex, it acts as a tissue-specific anchor between histone acetylations and methylations and chromatin remodeling. It thereby probably plays an essential role in heart and skeletal muscle development. Belongs to the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferativ |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The immunogen for anti-DPF3 antibody: synthetic peptide derected towards the N terminal of human DPF3 |
| Other Notes: | Synthetic peptide located within the following region: IHNPLKALGDQFYKEAIEHCRSYNS |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |