Anti-BDNF antibody produced in rabbit
SIGMA/SAB2108004
Synonym: Anti-ANON2; Anti-BULN2
Product Type: Product-on-demand
Immunohistochemistry Immunohistochemistry of BDNF in Ventral horn region of mouse spinal cord with BDNF antibody at 4-8 μg/mL
Immunoblotting Cell Type: fetal liver (Cat. No. SAB2108004). Lanes 1. Antibody dilution (concentration) 1 μg/mL in cell type fetal liver
Immunoblotting BDNF Antibody: Cat. No. SAB2108004: Western Blot analysis of BDNF in Human Liver cell lysates with BDNF Antibody at 1.0 μg/mL.
Western Blotting Western Blot of BDNF in Human ACHN with BDNF antibody at 1 μg/mL
Western Blotting Western Blot of BDNF in Mouse Brain with BDNF antibody at 1 μg/mL
Western Blotting Western Blot of BDNF in Mouse Pancreas with BDNF antibody at 1 μg/mL
Western Blotting Western Blot of BDNF in Mouse Testis with BDNF antibody at 1 μg/mL
Western Blotting Western Blot of BDNF in Human Ovary Tumor with BDNF antibody at 1 μg/mL
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 27kDa |
| NCBI accession no. | NM_001709   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | horse, rat, rabbit, dog, mouse, human, pig |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: suitable |
| immunohistochemistry: suitable | |
| UniProt accession no. | P23560 |
| Application: | Anti-BDNF antibody produced in rabbit has been used in immunohistochemistry and western blotting. |
| Biochem/physiol Actions: | Brain derived neurotrophic factor (BDNF) is involved in hippocampal function and verbal episodic memory in humans. Hence, variation in the BDNF gene expression alters these neurological functions. BDNF also plays a vital role in vascular function and participates in angiogenesis via the specific receptor tropomyosin-related kinase B (TrkB). It is involved in the pathogenesis of Alzheimer′s disease. ProBDNF interacts with p75 neurotrophin receptor, leading to long-term depression in the hippocampus. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The brain derived neurotrophic factor (BDNF) gene is mapped to human chromosome 11p14.1. BDNF is a member of the neurotrophin family of growth factors. The gene encodes a precursor protein, proBDNF. Mature BDNF (mBDNF) is synthesized by post-translational cleavage of proBDNF. Both proBDNF and mBDNF play crucial roles in cellular signaling. |
| Immunogen: | Synthetic peptide directed towards the middle region of human BDNF |
| Other Notes: | Synthetic peptide located within the following region: EWVTAADKKTAVDMSGGTVTVLEKV |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |