Anti-SGK1 antibody produced in rabbit
SIGMA/SAB2108342 - affinity isolated antibody
Product Type: Product-on-demand
Immunohistochemistry SGK1 Antibody: Cat. No. SAB2108342: Immunohistochemistry analysis of SGK1 in Human Pineal Tissue with SGK1 Antibody 5.0 μg/mL. Observed Staining: Cytoplasmic in cell bodies and processes of pinealocytes
Immunohistochemistry Immunohistochemistry of SGK1 in Human liver tissue with SGK1 antibody at 4-8 μg/mL
Immunoblotting Anti-SGK1, antibody produced in rabbit: Cat. No. SAB2108342: Immunoblotting analysis of SGK1 in HepG2 cell lysate with SGK1 anitbody at 1 μg/mL.
Western Blotting Western Blot of SGK1 in Mouse renal epithelial with SGK1 antibody at 1 μg/mL
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 49kDa |
| NCBI accession no. | NM_005627   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | guinea pig, rabbit, bovine, human, mouse, rat, dog, horse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: suitable |
| immunohistochemistry: suitable | |
| UniProt accession no. | O00141 |
| Biochem/physiol Actions: | The SGK1 (serum/glucocorticoid regulated kinase 1) gene is associated with a number of pathophysiological processes such as autophagy, ion transport, proliferation, metabolic, inflammation, syndrome and endoplasmic reticulum stress. Cellular dehydration, increased extracellular salt concentration, hormones such as glucocorticoids, mineralocorticoids, transforming growth factor β and mediators (cytokines) such as interleukin -6, stimulates SGK1 action. SGK1 gene expression is regulated by cellular signals involving cytosolic Ca2+, cyclic AMP (adenosine monophosphate), stress-activated protein kinase (SAPK2 (serine/threonine-protein kinase 2), p38 kinase), protein kinase C. Also, insulin and insulin-like growth factor, ERK (extracellular signal-regulated kinase) 1/2, and hepatic growth factor induces SGK1 transcription. Upregulation of SGK1 is observed in diabetes, liver cirrhosis, glomerulonephritis, dialysis and several tumors. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | SGK1 (serum/glucocorticoid regulated kinase 1) codes for a serine/threonine kinase and it is structurally and functionally homologous to kinases of AKT (protein kinase B) family. SGK1 is ubiquitously expressed in all tissues and is predominant in kidney. The SKG1 gene is mapped to human chromosome 6q23.2. |
| Immunogen: | Synthetic peptide directed towards the N terminal region of human SGK1 |
| Other Notes: | Synthetic peptide located within the following region: ANPSPPPSPSQQINLGPSSNPHAKP |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |