Anti-PCNA
SIGMA/SAB2108448 - IgG fraction of antiserum
Synonym: Anti-AIT
Product Type: Product-on-demand
Immunohistochemistry Anti-PCNA: Cat. No. SAB2108448: Immunohistochemistry of PCNA in Human Spleen tissue with PCNA antibody at 4-8 μg/mL.
Immunohistochemistry Immunohistochemistry of PCNA in Human NT-2 with PCNA antibody at 4-8 μg/mL
Immunohistochemistry Immunohistochemistry of PCNA in Human brain stem with PCNA antibody at 4-8 μg/mL
Western Blotting Western Blot of PCNA in Human NT-2 with PCNA antibody at 1 μg/mL
| accession no. | NM_002592 |
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5-1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 29 kDa |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | bovine, rat, dog, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: suitable |
| immunohistochemistry: suitable | |
| UniProt accession no. | P12004  |
| Application: | Anti-PCNA has been used in: • immunoblot (1:500) (1:1,000) (1:750) • far-western analysis (1:1000) • immunohistochemistry (1?:?50) (1:1000) • proliferating cell nuclear antigen (PCNA) overlay assay (1:1,000) |
| Biochem/physiol Actions: | Proliferating cell nuclear antigen (PCNA) participates in DNA replication and repair. It acts as an auxiliary factor of polymerase δ. It plays a key role in the maturation of Okazaki fragments. It can be used as a prognostic and diagnostic marker in chronic lymphoid leukemia (CLL). High expression of PCNA protein is seen in breast and duodenal cancers. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Proliferating cell nuclear antigen (PCNA), a nuclear protein, is expressed ubiquitously in mammals. This homotrimer protein is a doughnut-shaped molecule that is expressed at high levels in the thymus, bone marrow, fetal liver, and few cells of the small intestine and colon. PCNA is mainly located in the nucleus. The PCNA gene is a single-copy gene mapped to human chromosome 20p13. |
| Immunogen: | Synthetic peptide directed towards the C terminal region of human PCNA |
| Other Notes: | Synthetic peptide located within the following region: LNFFTKATPLSSTVTLSMSADVPLV |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |