Anti-BRD4
SIGMA/SAB2108521 - affinity isolated antibody
Synonym: Anti- HUNK1; Anti- HUNKI; Anti- MCAP; Anti-CAP
Product Type: Product-on-demand
Immunofluorescence Immunofluorescence of BRD4 in HeLa with BRD4 antibody at 4-8 μg/mL
Immunoblotting Cell Type: fetal thymus (Cat. No. SAB2108521). Lanes 1. Antibody dilution (concentration) 1.0 μg/mL in cell type fetal thymus
Western Blotting Western Blot of BRD4 in Human 293T Whole Cell with BRD4 antibody at 1 μg/mL
| accession no. | NM_014299 |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5-1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 80 kDa |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | bovine, rat, rabbit, human, horse, mouse, dog, guinea pig |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ChIP: suitable |
| immunofluorescence: suitable | |
| western blot: suitable | |
| UniProt accession no. | O60885-2  |
| Biochem/physiol Actions: | BRD4 is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting.The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | Synthetic peptide directed towards the C terminal region of human BRD4 |
| Other Notes: | Synthetic peptide located within the following region: EIEIDFETLKPSTLRELERYVTSCL |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |