Anti-GLS2
SIGMA/SAB2108573 - IgG fraction of antiserum
Synonym: Anti- GLS; Anti- LGA; Anti- MGC71567; Anti- hLGA; Anti-GA
Product Type: Product-on-demand
Immunohistochemistry Anti-GLS2: Cat. No. SAB2108573: Immunohistochemistry of GLS2 in Human Kidney tissue with GLS2 antibody at 4-8 μg/mL.
Immunoblotting Cell Type: HepG2 (Cat. No. SAB2108573). Lanes 1. Antibody dilution (concentration) 2.5 μg/mL in cell type HepG2
Western Blotting Western Blot of GLS2 in Human NCI-H226 with GLS2 antibody at 1 μg/mL
| accession no. | NM_013267 |
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5-1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 36 kDa |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | rat, horse, mouse, human, dog, pig |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: suitable |
| immunohistochemistry: suitable | |
| UniProt accession no. | Q9UI32  |
| Biochem/physiol Actions: | Glutaminase 2 (GLS2) is a mitochondrial phosphate-activated glutaminase that catalyses the hydrolysis of glutamine to stoichiometric amounts of glutamate and ammonia. This protein is functionally similar to the kidney glutaminase but is a little smaller in size. Originally thought to be liver-specific, this protein has been found in other tissues as well. GLS2 functions as a tumor protein p53 downstream target gene. GLS2 controls the functions of p53, in modulating energy metabolism and antioxidant defense. It might play a critical role in tumorigenesis. The protein negatively controls PI3K (phosphatidylinositol 3-kinase) /AKT (non-specific serine/threonine protein kinase) signalling pathway. GLS2 regulates the neuronal effects of tumor protein p73. The protein might have a pivotal role in radioresistance in cervical cancer patients. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Glutaminase 2 (GLS2) is located in mitochondria and nucleus. The gene is located on human chromosome 12q13. GLS2 protein is expressed in brain, pancreas, cancer cells and cells of the immune system. |
| Immunogen: | Synthetic peptide directed towards the middle region of human GLS2 |
| Other Notes: | Synthetic peptide located within the following region: FVGKEPSGLRYNKLSLNEEGIPHNP |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |