Anti-WT1
SIGMA/SAB2108752 - affinity isolated antibody
Synonym: Anti- AWT1; Anti- WAGR; Anti- WIT-2; Anti- WT33; Anti-GUD
Product Type: Product-on-demand
Immunohistochemistry Immunohistochemistry of WT1 in Human Testis with WT1 antibody at 4-8 μg/mL
Immunoblotting Anti-WT1, antibody produced in rabbit: Cat. No. SAB2108752: Immunoblotting analysis of WT1 in HT1080 cell lysate with WT1 anitbody at 1 μg/mL.
Western Blotting Western Blot of WT1 in Human Jurkat with WT1 antibody at 1 μg/mL
Western Blotting Western Blot of WT1 in Mouse Brain with WT1 antibody at 1 μg/mL
| accession no. | NM_024424 |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5-1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 56 kDa |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, rabbit, dog, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: suitable |
| immunohistochemistry: suitable | |
| UniProt accession no. | P19544  |
| Biochem/physiol Actions: | WT1 is a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system. WT1 has both oncogenic and tumor suppressor properties, and also acts as a transcription factor at the early organ developmental stage. WT1 regulates the mesenchyme and modulates the development of mesodermal organs. WT1 is known to cause kidney cancer or nephroblastoma in children. Mutations in WT1 causes diseases of urogenital system like Denys-Drash syndrome, Frasier syndrome. WT1 is being associated with haematological malignancies and solid tumours. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Wilms tumor 1 (WT1) gene is located on 11p13 in the human chromosome. |
| Immunogen: | Synthetic peptide directed towards the middle region of human WT1 |
| Other Notes: | Synthetic peptide located within the following region: DHLKTHTRTHTGEKPFSCRWPSCQK |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |