Anti-AIF1/IBA1 (Isoform 3) antibody produced in goat
SIGMA/SAB2500042 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-AIF-1; Anti-Allograft inflammatory factor 1; Anti-DASS-82G15.2; Anti-IBA1; Anti-IRT-1
MDL Number: MFCD02685938
Product Type: Chemical
Immunohistochemistry (2 μg/mL) staining of paraffin embedded Mouse Brain. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
Immunoblotting Anti-AIF1 / IBA1 (isoform 3): Cat. No. SAB2500042: (1 μg/mL) staining of Human Peripheral Blood Mononucleocyte lysate (35 μg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | goat |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| Quality Level | 100  |
| shipped in | dry ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| indirect ELISA: suitable | |
| western blot: suitable | |
| UniProt accession no. | P55008  |
| Application: | Anti-AIF1/IBA1 (Isoform 3) antibody produced in goat has been used in western blotting and immunofluorescence. |
| Biochem/physiol Actions: | AIF1 (allograft inflammatory factor 1) is an essential factor of macrophage chemotaxis. Expression of AIF1 regulates the proliferation of vascular smooth muscle cells by G-CSF (cytokine granulocyte-colony stimulating factor) autocrine expression. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | AIF1 (allograft inflammatory factor 1) is a calcium-binding, cytoplasmic protein that has 143-amino acids. It is also known as IBA1 (ionized calcium-binding adapter molecule 1). It is expressed in the cells of the monocyte/macrophage lineage, as well as in microglial and dendritic cells. It is located on human chromosome 6p21.3. |
| Immunogen: | Peptide with sequence C-NKQFLDDPKYSSDED from the internal region of the protein sequence according to NP_001614.3. |
| Physical form: | Supplied at 0.5 mg/mL in Tris saline with 0.02% sodium azide and 0.5% bovine serum albumin. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |