Anti-CD32/FCGR2B antibody produced in goat
SIGMA/SAB2500218 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-FCG2; Anti-FCGR2; Anti-IGFR2
Product Type: Chemical
EB06695 Negative Control showing staining of paraffin embedded Human Placenta, with no primary antibody.
EB06695 (8μg/ml) staining of paraffin embedded Human Placenta. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB06695 (1μg/ml) staining of Daudi cell lysate (RIPA buffer, (35μg protein in RIPA buffer). Detected by chemiluminescence.
EB06695 Immunofluorescence analysis of paraformaldehyde fixed THP-1 cells immobilized on ShifixTM coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Immunoblotting Anti-CD32 / FCGR2B: Cat. No. SAB2500218: (0.3 μg/mL) staining of K562 lysate (35 μg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunoblotting Anti-CD32 / FCGR2B: Cat. No. SAB2500218: HEK293 overexpressing CD32 (RC211982) and probed with Cat. No. SAB2500218 (mock transfection in first lane), tested by Origene.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | goat |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| Quality Level | 100  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| immunohistochemistry: suitable | |
| indirect ELISA: suitable | |
| western blot: suitable | |
| UniProt accession no. | P31994  |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Peptide with sequence C-PDALEEPDDQNRI from the C Terminus of the protein sequence according to NP_003992.3; NP_001002273.1; NP_001002274.1; NP_001002275.1;. |
| Physical form: | Supplied at 0.5 mg/mL in Tris saline with 0.02% sodium azide and 0.5% bovine serum albumin. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H315 - H319 |
| Precautionary statements | P305 + P351 + P338 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |