Anti-GAPDH, C-Terminal antibody produced in goat

SIGMA/SAB2500450 - affinity isolated antibody, buffered aqueous solution

Synonym: G3PD; GAPD; Glyceraldehyde-3-phosphate dehydrogenase; HEL-S-162eP; HGNC:4141; MGC88685; aging-associated gene 9 protein; epididymis secretory sperm binding protein Li 162eP; peptidyl-cysteine S-nitrosylase GAPDH

Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-SAB2500450-100UG	100 µg	$628.00 1/EA	Add To Favorites

Immunohistochemistry Anti-GAPDH (C Terminus): Cat. No. SAB2500450: (0.3 μg/mL) staining of paraffin embedded Human Tonsil. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. A) Staining of germinal centre cells. B) Staining of endothelial cells.

EB06377 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

EB06377 (0.5μg/ml) staining of HeLa (A), HEK293 (B), LNCaP (C) and A549 (D) cell lysate (35μg protein in RIPA buffer). Detected by chemiluminescence.

EB06377 (0.5μg/ml) staining of Human Liver lysate (35μg protein in RIPA buffer). Detected by chemiluminescence.

Properties
Descriptions
Safety Info.
Basic Data

antibody form	affinity isolated antibody
antibody product type	primary antibodies
biological source	goat
clone	polyclonal
conjugate	unconjugated
form	buffered aqueous solution
Quality Level	100
shipped in	dry ice
species reactivity	mouse, human, rat
species reactivity (predicted by homology)	canine, pig
storage temp.	−20°C
target post-translational modification	unmodified
technique(s)	immunofluorescence: suitable
	immunohistochemistry: suitable
	indirect ELISA: suitable
	western blot: suitable
UniProt accession no.	P04406

Application:	Recommended concentration: Western Blot 0.01-0.5ug/uL Immunohistochemistry 5 ug/mL Immunofluorescence 5 ug/mL Optimal dilutions/concentrations should be determined by the end user. Anti-GAPDH, C-Terminal antibody produced in goat has been used in single cell western blot analysis.
Biochem/physiol Actions:	Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) plays an important role in DNA repair. It is essential for the sixth step of glycolysis. It participates in transcriptional and posttranscriptional gene regulation. It also controls intracellular membrane trafficking and cell death. Accumulation of GAPDH results in mitochondrial dysfunction. Phosphorylated GAPDH associates with cytoskeletal elements and controls microtubule dynamics in the early secretory pathway. GAPDH is also a component of the functional GAIT (interferon-γ-activated inhibitor of translation) mRNP (messenger ribonucleoprotein). GAPDH expression is dysregulated during melanoma progression.
Disclaimer:	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Features and Benefits:	Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.
General description:	Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a multifunctional protein and is encoded by the gene mapped to human chromosome 12p13. It is basically called as a glycolytic enzyme.
Immunogen:	Peptide with sequence C-HQVVSSDFNSDT from the C Terminus of the protein sequence according to NP_002037.2.
Physical form:	Supplied at 0.5 mg/mL in Tris saline with 0.02% sodium azide and 0.5% bovine serum albumin.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 2

Storage Temp.	−20°C
UNSPSC	12352203

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