Anti-Smooth Muscle α-Actin antibody produced in goat
SIGMA/SAB2500963 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-ACTA2; Anti-
Product Type: Chemical
SAB2500963 (0.3μg/ml) staining of Human (A) and (0.5ug/ml) Mouse (B) and Rat (C) Duodenum lysate (35μg protein in RIPA buffer). Detected by chemiluminescence.
(1μg/ml) staining of Human Heart (A), (0.1ug/ml) Mouse Duodenum (B) and (0.03ug/ml) Rat Heart (C) lysate (35μg protein in RIPA buffer). Detected by chemiluminescence.
(1μg/ml) staining of HeLa (A) and NIH3T3 (B) cell lysate (35μg protein in RIPA buffer). Detected by chemiluminescence.
Western Blotting (1μg/ml) staining of HeLa (A) and (2ug/ml) NIH3T3 (B) cell lysate (35μg protein in RIPA buffer). Detected by chemiluminescence.
Western Blotting (1μg/ml) staining of Mouse Duodenum lysate (35μg protein in RIPA buffer). Detected by chemiluminescence.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | goat |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| Quality Level | 100  |
| shipped in | dry ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: suitable |
| western blot: suitable | |
| UniProt accession no. | P62736  |
| Application: | Goat polyclonal anti-smooth muscle α-Actin antibody is used to tag smooth muscle α-actin (ACTA2) for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques. It is used as a probe to determine the presence and roles of smooth muscle α-actin in smooth muscle structure and as a marker of myofibroblast formation. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures in all cell types. It varies in amount related to the type of differentiation and to the functional state of cells and tissues. Actin can be found in two different forms of aggregation, the globular or the fibrillar state, and at least six distinct isoforms occur in vertebrates. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three α actins (skeletal, cardiac, smooth), one β actin (β-non-muscle) and two γ actins (γ smooth muscle and γ non-muscle). Difficulties have been encountered in producing polyclonal antibodies due to the highly conserved nature of actin. |
| General description: | Goat polyclonal anti-smooth muscle α-Actin antibody reacts with canine, human, mouse, and rat smooth muscle α-actins (ACTA2). |
| Immunogen: | Peptide with sequence EEEDSTALVC from the N Terminus of the protein sequence according to NP_001604.1. |
| Physical form: | Supplied at 0.5 mg/mL in Tris saline with 0.02% sodium azide and 0.5% bovine serum albumin. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |