Anti-Uncoupling Protein 2/UCP2 antibody produced in goat
SIGMA/SAB2501087 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-SLC25A8; Anti-UCPH; Anti-Uncoupling protein 2
Product Type: Chemical
Immunofluorescence Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor™ 488 secondary antibody (2ug/ml), showing Mitochondrial staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor™ 488 secondary antibody (2ug/ml).
Western Blotting (1μg/ml) staining of Rat Adipose (A) and Mouse Spleen (B) lysate (35μg protein in RIPA buffer). Detected by chemiluminescence.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | goat |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| Quality Level | 100  |
| shipped in | dry ice |
| species reactivity | mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| indirect ELISA: suitable | |
| western blot: suitable | |
| UniProt accession no. | P70406  |
| Application: | Anti-uncoupling protein 2/UCP2 antibody has been used in immunoblotting. |
| Biochem/physiol Actions: | Uncoupling protein 2 (UCP2) controls energy metabolism and decreases the production of reactive oxygen species and guards against reperfusion damage. It plays a vital role in uncoupling electron transport from adenosine triphosphate (ATP) formation. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Uncoupling protein 2 (UCP2) is an inner mitochondrial membrane protein. It expressed in various regions of the brain. In non-human primates, it is present in the hypothalamic nuclei, pituitary gland, brainstem, ventral tegmental area and substantia nigra. It is located on human chromosome 11q13. |
| Immunogen: | Peptide with sequence C-DSVKQFYTKGSEH from the internal region of the protein sequence according to NP_003346.2. |
| Physical form: | Supplied at 0.5 mg/mL in Tris saline with 0.02% sodium azide and 0.5% bovine serum albumin. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |