Anti-IGF2BP1 antibody produced in goat
SIGMA/SAB2501343 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-CRD-BP; Anti-CRDBP; Anti-IGF2BP1; Anti-IMP-1; Insulin-like growth factor 2 mRNA binding protein 1
Product Type: Product-on-demand
SAB2501343 (3.75μg/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
SAB2501343 Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by ALEXA FLUOR™ 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by ALEXA FLUOR™ 488 secondary antibody.
SAB2501343 (0.1μg/ml) staining of Caco-2 (A), (0.3μg/ml) K562 (B), nuclear HepG2 (C) and (1μg/ml) nuclear NIH3T3 (D) cell lysate (35μg protein in RIPA buffer). Detected by chemiluminescence.
SAB2501343 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by ALEXA FLUOR™ 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by ALEXA FLUOR™ 488 secondary antibody (2ug/ml).
SAB2501343 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by ALEXA FLUOR™ 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by ALEXA FLUOR™ 488 secondary antibody (2ug/ml).
Immunoblotting Anti-IGF2BP1 Cat. No. SAB2501343: (0.3 μg/mL) staining of K562 lysate (35 μg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | goat |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| Quality Level | 100  |
| shipped in | dry ice |
| species reactivity | canine, rat, human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: suitable |
| immunohistochemistry: suitable | |
| western blot: suitable | |
| UniProt accession no. | Q9NZI8  |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Peptide with sequence EKVFAEHKISYSGQ, from the internal region of the protein sequence according to NP_006537.3; NP_001153895.1 |
| Physical form: | Supplied at 0.5 mg/mL in 20mM Tris (pH 7.3) and 150mM NaCl with 0.02% sodium azide and 0.5% bovine serum albumin. |
| Hazard Codes | Xn |
| Risk Statements | 22 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |