Anti-NS3 antibody produced in rabbit
SIGMA/SAB2700181 - affinity isolated antibody, buffered aqueous solution
Synonym: Dengue virus 2 Nonstructural protein NS3; Non-structure protein 3
Product Type: Product-on-demand
Immunofluorescence NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis. Samples: BHK-21 cells mock (left) and infected with Dengue virus 1 (right) were fixed in MeOH. Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody (SAB2700181) diluted at 1:2000. Blue: Hoechst 33342 staining.
Immunofluorescence NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis. Samples: BHK-21 cells mock (left) and infected with Dengue virus 4 (right) were fixed in MeOH. Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody (SAB2700181) diluted at 1:2000. Blue: Hoechst 33342 staining.
Immunofluorescence Immunofluorescence analysis of paraformaldehyde-fixed BHK-21 cell, using NS3 (Dengue virus 2)(SAB2700181) antibody at 1:2000 dilution.
Immunofluorescence NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis. Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH. Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody (SAB2700181) diluted at 1:2000. Blue: Hoechst 33342 staining.
Immunofluorescence NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis. Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH. Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody (SAB2700181) diluted at 1:2000. Blue: Hoechst 33342 staining.
Western Blotting Sample (20 μg of whole cell lysate) A: BHK-21 B: Dengue virus 2 infect BHK-21 C: JEV infect BHK-21 7.5% SDS PAGE SAB2700181 diluted at 1:2000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blotting NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein by western blot analysis. A. 30 μg BHK-21 whole cell extract B. 30 μg whole cell extract of Dengue virus type 1 infected BHK-21 cells C. 30 μg whole cell extract of Dengue virus type 2 infected BHK-21 cells D. 30 μg whole cell extract of Dengue virus type 3 infected BHK-21 cells E. 30 μg whole cell extract of Dengue virus type 4 infected BHK-21 cells 7.5% SDS-PAGE NS3 (Dengue virus) antibody (SAB2700181) dilution: 1:2000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blotting Non-infected (-) and infected (+) BHK-21 whole cell extracts (15 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dengue virus NS3 protein antibody (SAB2700181) diluted at 1:2000.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| NCBI accession no. | NP_739587   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | Dengue virus |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: suitable |
| immunofluorescence: suitable | |
| immunohistochemistry: suitable | |
| western blot: 500-3000 |
| Application: | Suggested starting dilutions are as follows: FACS: Assay-dependent dilution, ICC/IF: 1:100-1:2000, IHC: Assay-dependent dilution, IHC-P: Assay-dependent dilution, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. Anti-NS3 antibody produced in rabbit has been used in flow cytometry. |
| Biochem/physiol Actions: | Non-structure protein 3 (NS3) along with NS2B play a vital role in proteolytic cleavage of dengue virus (DEN) non-structural proteins. NS3 also acts as a RNA-stimulated nucleoside-triphosphatase (NTPase)/RNA helicase and a 5′-RNA triphosphatase (RTPase). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Non-structure protein 3 (NS3), also known as dengue virus type 2 (DEN2), belongs to the Flaviviridae family. NS3 is a multifunctional protein with a molecular mass of about 69 kDa. It contains serine protease domain (NS3-pro) and it needs the hydrophilic domain of NS2B (NS2BH) for activation. |
| Immunogen: | Recombinant protein fragment contain a sequence corresponding to a region within amino acids 354 and 568 of NS3 (Dengue virus 2) according to NP_739587 |
| Physical form: | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 - H412 |
| Precautionary statements | P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |