Anti-NS1 antibody produced in rabbit
SIGMA/SAB2700186 - affinity isolated antibody, buffered aqueous solution
Synonym: Dengue virus 2 Nonstructural protein NS1; Non-structure protein 1
Product Type: Product-on-demand
Immunofluorescence NS1 (Dengue virus) antibody detects NS1 (Dengue virus) protein at cytoplasm by immunofluorescent analysis. Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH. Green: NS1 (Dengue virus) protein stained by NS1 (Dengue virus) antibody (SAB2700186) diluted at 1:2000. Blue: Hoechst 33342 staining.
Immunofluorescence NS1 (Dengue virus) antibody detects NS1 (Dengue virus) protein at cytoplasm by immunofluorescent analysis. Samples: BHK-21 cells mock (left) and infected with Dengue virus 1 (right) were fixed in MeOH. Green: NS1 (Dengue virus) protein stained by NS1 (Dengue virus) antibody (SAB2700186) diluted at 1:2000. Blue: Hoechst 33342 staining.
Immunofluorescence NS1 (Dengue virus) antibody detects NS1 (Dengue virus) protein at cytoplasm by immunofluorescent analysis. Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH. Green: NS1 (Dengue virus) protein stained by NS1 (Dengue virus) antibody (SAB2700186) diluted at 1:2000. Blue: Hoechst 33342 staining.
Immunofluorescence NS1 (Dengue virus) antibody detects NS1 (Dengue virus) protein at cytoplasm by immunofluorescent analysis. Samples: BHK-21 cells mock (left) and infected with Dengue virus 4 (right) were fixed in MeOH. Green: NS1 (Dengue virus) protein stained by NS1 (Dengue virus) antibody (SAB2700186) diluted at 1:2000. Blue: Hoechst 33342 staining.
Immunofluorescence Immunofluorescence analysis of paraformaldehyde-fixed BHK-21 cell, using NS1 (Dengue virus 2)(SAB2700186) antibody at 1:2000 dilution.
Western Blotting Sample (20 μg of whole cell lysate) A: BHK-21 B: Dengue virus 2 infect BHK-21 C: JEV infect BHK-21 12% SDS PAGE SAB2700186 diluted at 1:2000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blotting NS1 (Dengue virus) antibody detects NS1 (Dengue virus) protein by western blot analysis. A. 66 μg BHK-21 whole cell extract B. 66 μg whole cell extract of Dengue virus type 1 infected BHK-21 cells C. 20 μg whole cell extract of Dengue virus type 2 infected BHK-21 cells D. 10 μg whole cell extract of Dengue virus type 3 infected BHK-21 cells E. 66 μg whole cell extract of Dengue virus type 4 infected BHK-21 cells 10% SDS-PAGE NS1 (Dengue virus) antibody (SAB2700186) dilution: 1:3000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blotting NS1 (Dengue virus ) antibody detects NS1 (Dengue virus ) protein by Western blot analysis. Un-infected (-) and infected (+, DENV2 infection for ) BHK-21 whole cell extracts (20 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NS1 (Dengue virus ) antibody (SAB2700186) diluted by 1:2000.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| NCBI accession no. | NP_739584   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | Dengue virus |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: suitable |
| flow cytometry: suitable | |
| immunofluorescence: suitable | |
| western blot: 500-3000 |
| Application: | Suggested starting dilutions are as follows: ELISA: Assay-dependent dilution, FACS: Assay-dependent dilution, ICC/IF: 1:100-1:2000, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Recombinant protein fragment contain a sequence corresponding to a region within amino acids 117 and 301 of NS1 (Dengue virus 2) according to NP_739584 |
| Physical form: | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 - H412 |
| Precautionary statements | P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |