Anti-ANGPT2 antibody produced in rabbit
SIGMA/SAB2700230 - affinity isolated antibody, buffered aqueous solution
Synonym: AGPT2; ANG2; ANGPT2
Product Type: Product-on-demand
Immunohistochemistry Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Angiopoietin 2 (SAB2700230) antibody at 1:500 dilution.
Immunofluorescence Immunofluorescence analysis of methanol-fixed A549, using Angiopoietin 2 (SAB2700230) antibody at 1:500 dilution.
Western Blotting Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Angiopoietin 2 antibody (SAB2700230) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blotting Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Angiopoietin 2 antibody (SAB2700230) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blotting Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Angiopoietin 2 antibody (SAB2700230) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| NCBI accession no. | NP_001138   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunofluorescence: suitable |
| immunohistochemistry: suitable | |
| western blot: 500-3000 | |
| UniProt accession no. | O15123 |
| Application: | Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IHC-P: 1:100-1:1000, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Biochem/physiol Actions: | The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq] |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Recombinant fragment corresponding to a region within amino acids 1 and 308 of Angiopoietin 2 according to NP_001138 |
| Physical form: | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 - H412 |
| Precautionary statements | P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |