Anti-GPC1 antibody produced in rabbit
SIGMA/SAB2700282 - affinity isolated antibody, buffered aqueous solution
Synonym: FLJ38078; GPC1; glypican
Product Type: Product-on-demand
Immunohistochemistry Immunohistochemical microscopy analysis of paraffin-embedded human normal pancreas (left) or pancreatic adenocarcinoma (grade II) (right) tissues using Glypican-1 antibody [N3C3] (SAB2700282) at a 1:250 dilution.
Immunofluorescence Glypican 1 antibody [N3C3] detects Glypican 1 protein at cytoplasm by immunofluorescent analysis. Sample: MCF7 cells were fixed in ice-cold MeOH for 5 min. Green: Glypican 1 protein stained by Glypican 1 antibody [N3C3] (SAB2700282) diluted at 1:500. Blue: Hoechst 33342 staining.
Western Blotting Untreated (-) and treated (+) A431 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (SAB2700282) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blotting Untreated (-) and treated (+) A431 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (SAB2700282) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blotting Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (SAB2700282) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blotting Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Glypican 1 antibody [N3C3] (SAB2700282) diluted at 1:500 and competitor′s antibody (sc-365000) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blotting U87-MG whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (SAB2700282) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blotting Mouse tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (SAB2700282) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blotting U87-MG whole cell extract and conditioned medium (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (SAB2700282) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blotting Untreated (–) and treated (+) MDA-MB-231 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (SAB2700282) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunoprecipitation Immunoprecipitation of Glypican 1 protein from MCF-7 whole cell extracts using 5 μg of Glypican 1 antibody [N3C3] (SAB2700282). Western blot analysis was performed using Glypican 1 antibody [N3C3] (SAB2700282). EasyBlot anti-Rabbit IgG was used as a secondary reagent.
ELISA Glypican 1 antibody [N3C3] (SAB2700282) detects Glypican 1 protein by flow cytometry analysis. Sample: A431 cell. Black: Unlabelled sample was used as a control. Red: Glypican 1 antibody [N3C3] (SAB2700282) dilution: 1:100. Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.
Flow Cytometry An ELISA plate is coated with MCF-7 and A431 cells. The coated cells are detected with Glypican 1 antibody (SAB2700282) at concentration ranged from 7.5 to 240 ng/mL.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| application(s) | research pathology |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| NCBI accession no. | NP_002072   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: suitable |
| flow cytometry: suitable | |
| immunofluorescence: suitable | |
| immunohistochemistry: suitable | |
| immunoprecipitation (IP): suitable | |
| western blot: 500-3000 | |
| UniProt accession no. | P35052 |
| Application: | Anti-GPC1 antibody produced in rabbit has been used in flow cytometry and immunostaining. |
| Biochem/physiol Actions: | Glypican 1 (GPC1) plays an important role in cell growth, differentiation and morphogenesis. It also plays a role in cell migration, adhesion, anti-coagulation, lipoprotein metabolism and modulation of growth factors. GPC1 exhibits chaperone-like activity by restoring the binding activity of oxidized VEGF165 (vascular endothelial growth factors-165). Overexpression of GPC1 results in over accumulation of β-amyloid protein (Aβ) in the Alzheimer′s disease (AD) brain. Mutation in this gene is associated with Niemann-Pick C1 disease. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Glypican 1 (GPC1) is located at 2q37.3 on the human chromosome. It belongs to the family of glycosylphosphatidylinosi |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human Glypican 1. |
| Physical form: | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 - H412 |
| Precautionary statements | P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |