Anti-SEC61A1 antibody produced in rabbit
SIGMA/SAB2700328 - affinity isolated antibody, buffered aqueous solution
Synonym: HSEC61; SEC61; SEC61A; SEC61A1
Product Type: Product-on-demand
Immunohistochemistry Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using SEC61A1(SAB2700328) antibody at 1:500 dilution.
Immunofluorescence Immunofluorescence analysis of methanol-fixed A431, using SEC61A1(SAB2700328) antibody at 1:200 dilution.
Western Blotting SEC61A1 antibody detects SEC61A1 protein by Western blot analysis. K562 whole cell extracts and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SEC61A1 antibody (SAB2700328) diluted at 1:1000.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| NCBI accession no. | NP_037468   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| immunohistochemistry: suitable | |
| western blot: 500-3000 | |
| UniProt accession no. | P61619 |
| Application: | Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IHC-P: 1:100-1:1000, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Biochem/physiol Actions: | The protein encoded by this gene belongs to the SECY/SEC61- alpha family. It appears to play a crucial role in the insertion of secretory and membrane polypeptides into the endoplasmic reticulum. This protein found to be tightly associated with membrane-bound ribosomes, either directly or through adaptor proteins. This gene encodes an alpha subunit of the heteromeric SEC61 complex, which also contains beta and gamma subunits. [provided by RefSeq] |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Synthetic peptide containing a sequence corresponding to a region within amino acids 413 and 426 (P61619) of SEC61A1 according to NP_037468 |
| Physical form: | 1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 - H412 |
| Precautionary statements | P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |