Anti-GPER (C-terminal) antibody produced in rabbit
SIGMA/SAB2700363 - affinity isolated antibody, buffered aqueous solution
Synonym: CEPR; CMKRL2; DRY12; GPER
Product Type: Product-on-demand
Immunohistochemistry Immunohistochemical analysis of paraffin-embedded human endometrial, using GPR30(SAB2700363) antibody at 1:150 dilution.
Immunohistochemistry Immunohistochemical analysis of paraffin-embedded human endometrial, using GPR30(SAB2700363) antibody at 1:150 dilution.
Western Blotting GPR30 antibody [C2C3], C-term detects GPR30 protein by western blot analysis. A. 50 μg 293T whole cell lysate/extract B. 50 μg whole cell lysate/extract of GFP-human GPR30-transfected 293T cells (No boiling) C. 50 μg whole cell lysate/extract of GFP-human GPR30 and GPR30 siRNA-transfected 293T cells (No boiling) 5% SDS-PAGE GPR30 antibody [C2C3], C-term (SAB2700363) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blotting Sample (40ug of whole cell lysate) A: RL952 cell B: GPR30 shRNA-transfected RL952 cell SAB2700363 diluted at 1:10,000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blotting Sample (30 μg of whole cell lysate) A: H1299 B: HeLa 10% SDS PAGE SAB2700363 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| NCBI accession no. | NP_001035055   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunofluorescence: suitable |
| immunohistochemistry: suitable | |
| western blot: 1:500-1:3,000 |
| Application: | Suggested starting dilutions are as follows: IHC-P: 1:100-1:1000, WB: 1:1000-1:10000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Biochem/physiol Actions: | This gene is a member of the G-protein coupled receptor 1 family and encodes a multi-pass membrane protein that localizes to the endoplasmic reticulum. The protein binds estrogen, resulting in intracellular calcium mobilization and synthesis of phosphatidylinositol 3,4,5-trisphosphate in the nucleus. This protein therefore plays a role in the rapid nongenomic signaling events widely observed following stimulation of cells and tissues with estrogen. Alternate transcriptional splice variants which encode the same protein have been characterized. [provided by RefSeq] |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Synthetic peptide corresponding to a region within amino acids 307 and 375 of GPR30 according to NP_001035055 |
| Physical form: | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 - H412 |
| Precautionary statements | P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |