Anti-LRPPRC antibody produced in rabbit
SIGMA/SAB2700419 - affinity isolated antibody, buffered aqueous solution
Synonym: CLONE-23970; GP130; LRP130; LRPPRC
Product Type: Product-on-demand
Immunohistochemistry LRP130 antibody [N3C2], Internal detects LRP130 protein at cytoplasm in human colon cancer by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. LRP130 antibody [N3C2], Internal (SAB2700419) diluted at 1:100.
Immunohistochemistry Immunohistochemical analysis of paraffin-embedded SW480 Xenograft, using LRP130 (SAB2700419) antibody at 1:100 dilution.
Immunofluorescence LRP130 antibody [N3C2], Internal detects LRP130 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: LRP130 protein stained by LRP130 antibody [N3C2], Internal (SAB2700419) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Western Blotting Sample (30 μg of whole cell lysate) A: H1299 B: HeLa 5% SDS PAGE SAB2700419 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blotting Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with LRP130 antibody [N3C2], Internal (SAB2700419) diluted at 1:1000.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| NCBI accession no. | NP_573566   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| immunohistochemistry: suitable | |
| western blot: 1000-10000 | |
| UniProt accession no. | P42704 |
| Application: | Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IHC-P: 1:100-1:1000, WB: 1:1000-1:10000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Biochem/physiol Actions: | This gene encodes a protein that is leucine-rich and is thought to play a role in regulating the interaction of the cytoskeleton with a variety of cellular processes. Mutations in this gene are associated with the French-Canadian type of Leigh syndrome. Transcripts ranging in size from 4.8 to 7.0 kb which result from alternative polyadenylation have been reported for this gene. [provided by RefSeq] |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Recombinant fragment corresponding to a region within amino acids 611 and 937 of LRP130 according to NP_573566 |
| Physical form: | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 - H412 |
| Precautionary statements | P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |