Anti-GPX1 antibody produced in rabbit
SIGMA/SAB2700534 - affinity isolated antibody, buffered aqueous solution
Synonym: GPX1; GSHPX1; MGC14399; MGC88245
Product Type: Product-on-demand
Immunohistochemistry Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using GPX1(SAB2700534) antibody at 1:100 dilution.
Immunofluorescence Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Glutathione peroxidase 1(SAB2700534) antibody at 1:200 dilution.
Western Blotting Sample (30 μg of whole cell lysate) A: Molt-4 B: Raji 12% SDS PAGE SAB2700534 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blotting GPX1 antibody detects GPX1 protein by western blot analysis. A. 30 μg GL261 whole cell lysate/extract lysate/extract B. 30 μg C8D30 whole cell lysate/extract C. 30 μg NIH-3T3 whole cell lysate/extract D. 30 μg Raw264.7 whole cell lysate/extract E. 30 μg C2C12 whole cell lysate/extract 12% SDS-PAGE GPX1 antibody (SAB2700534) dilution: 1:2000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blotting GPX1 antibody detects GPX1 protein by western blot analysis. A.50 μg rat liver lysate/extract 12% SDS-PAGE GPX1 antibody (SAB2700534) dilution: 1:10,000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| NCBI accession no. | NP_000572   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, alligator, mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| immunohistochemistry: suitable | |
| western blot: 1000-10000 | |
| UniProt accession no. | P07203 |
| Application: | Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IHC-P: 1:100-1:1000, WB: 1:1000-1:10000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Biochem/physiol Actions: | This gene encodes a member of the glutathione peroxidase family. Glutathione peroxidase functions in the detoxification of hydrogen peroxide, and is one of the most important antioxidant enzymes in humans. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by UGA, that normally functions as a translation termination codon. In addition, this protein is characterized in a polyalanine sequence polymorphism in the N-terminal region, which includes three alleles with five, six or seven alanine (ALA) repeats in this sequence. The allele with five ALA repeats is significantly associated with breast cancer risk. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq] |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Synthetic peptide corresponding to a region within amino acids 127 and 203 of GPX1 according to NP_000572 |
| Physical form: | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 - H412 |
| Precautionary statements | P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |