Anti-TET3 (C-terminal) antibody produced in rabbit
SIGMA/SAB2700682 - affinity isolated antibody, buffered aqueous solution
Synonym: KIAA0401; MGC22014; TET3
Product Type: Product-on-demand
Immunohistochemistry TET3 antibody [C3], C-term detects TET3 protein at nucleus on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. TET3 antibody [C3], C-term (SAB2700682) dilution: 1:500.
Immunofluorescence TET3 antibody [C3], C-term detects TET3 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TET3 protein stained by TET3 antibody [C3], C-term (SAB2700682) diluted at 1:400. Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
Western Blotting TET3 antibody [C3], C-term detects TET3 protein by Western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg whole cell lysate/extract of Flag-human TET3 transfected 293T cells 5 % SDS-PAGE TET3 antibody [C3], C-term (SAB2700682) dilution: 1:5000
Immunoprecipitation TET3 antibody immunoprecipitates TET3 protein in IP experiments. IP Sample: cell lysate/extract of TET3 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of TET3 by 2 μg of TET3 antibody (SAB2700682) 5% SDS-PAGE The immunoprecipitated TET3 protein was detected by TET3 antibody (SAB2700682) diluted at 1:1000. EasyBlot anti-Rabbit IgG was used as a secondary reagent.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| NCBI accession no. | AAH22243   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | Xenopus, human, bovine, mouse |
| storage temp. | −20°C |
| technique(s) | ChIP: suitable |
| immunofluorescence: suitable | |
| immunohistochemistry: 1:100-1:1000 | |
| immunoprecipitation (IP): suitable | |
| western blot: 1000-10000 | |
| UniProt accession no. | O43151 |
| Application: | Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IHC-P: 1:100-1:1000, IP: 1:500-1:1000, WB: 1:1000-1:10000, IHC-Wm: Assay-dependent dilution, ChIP assay: Assay-dependent dilution. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Biochem/physiol Actions: | Members of the ten-eleven translocation (TET) gene family, including TET3, play a role in the DNA methylation process (Langemeijer et al., 2009 [PubMed 19923888]).[supplied by OMIM] |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Recombinant fragment corresponding to a region within amino acids 1456 and 1660 of TET3 according to AAH22243 |
| Physical form: | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 - H412 |
| Precautionary statements | P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |