Monoclonal Anti-TET1 antibody produced in mouse
SIGMA/SAB2700730 - clone GT1462, affinity isolated antibody, buffered aqueous solution
Synonym: CXXC6; FLJ10839; FLJ41442; TET1
Product Type: Product-on-demand
Immunohistochemistry TET1 antibody [GT1462] detects TET1 protein at nucleus on HeLa xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HeLa xenograft. TET1 antibody [GT1462] (SAB2700730) dilution: 1:100.
Immunofluorescence TET1 antibody [GT1462] detects TET1 protein at nucleus by immunofluorescent analysis. Sample: TET1-transfected (right) or untransfected (left) 293T cells were fixed in 4% paraformaldehyde for 15 min. Green: TET1 protein stained by TET1 antibody (SAB2700730) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Western Blotting TET1 antibody [GT1462] detects TET1 protein by western blot analysis. A. 50 μg whole cell lysate/extract from 293T cells transfected with scramble siRNA B. 50 μg whole cell lysate/extract from TET1-knockdowned 293T cells 6% SDS-PAGE TET1 antibody [GT1462] (SAB2700730) dilution: 1:500 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Western Blotting NT2D1 whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [GT1462] (SAB2700730) diluted at 1:500.
Western Blotting TET1 antibody detects TET1 protein by Western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg whole cell lysate/extract of DDDDK-human TET1-transfected 293T cells 5 % SDS-PAGE TET1 antibody (SAB2700730) dilution: 1:500
Immunoprecipitation Immunoprecipitation of TET1 protein from NT2D1 whole cell extracts using 5 μg of TET1 antibody [GT1462] (SAB2700730). Western blot analysis was performed using TET1 antibody [GT1462] (SAB2700730).
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | GT1462, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2 |
| NCBI accession no. | NP_085128   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ChIP: suitable |
| immunofluorescence: suitable | |
| immunohistochemistry: 1:100-1:1000 | |
| immunoprecipitation (IP): suitable | |
| western blot: 500-3000 | |
| UniProt accession no. | Q8NFU7 |
| Application: | Recombinant protein encompassing a sequence within the center region of human TET1. The exact sequence is proprietary. |
| Application: | Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IHC-P: 1:100-1:1000, IP: 1:100-1:500, WB: 1:500-1:3000, ChIP assay: Assay-dependent dilution. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Biochem/physiol Actions: | Tet methylcytosine dioxygenase 1 (TET1) functions as a 5-methylcytosine (5mC) hydroxylase. It converts 5-methylcytosine to 5-hydroxymethylcytosine, which is crucial for active DNA demethylation in the adult brain. The protein has been linked to the progression of colorectal cancer. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Tet methylcytosine dioxygenase 1 (TET1) is a protein that belongs to the TET family and contains a zinc-binding CXXC domain. It is expressed in embryonic stem cells. The TET1 gene is localized on human chromosome 10q21.3. |
| Other Notes: | For in vitro laboratory use only. Not for any clinical, therapeutic or diagnostic use in humans or animals. Not for animal or human consumption. |
| Physical form: | Phosphate-buffered saline, no preservative added. |
| Preparation Note: | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |