Anti-DKK3 antibody produced in rabbit
SIGMA/SAB2700747 - affinity isolated antibody, buffered aqueous solution
Synonym: DKK3; REIC; RIG
Product Type: Product-on-demand
Immunohistochemistry DKK3 antibody [N1C3] detects DKK3 protein at cytoplasm on human normal liver by immunohistochemical analysis. Sample: Paraffin-embedded human normal liver. DKK3 antibody [N1C3] (SAB2700747) diluted at 1:500.
Immunofluorescence Immunofluorescence analysis of methanol-fixed A549, using DKK3(SAB2700747) antibody at 1:500 dilution.
Western Blotting Sample (30 μg of whole cell lysate) A: A549 10% SDS PAGE SAB2700747 diluted at 1:1000
Western Blotting Sample (50 μg of whole cell lysate) A: Mouse brain 10% SDS PAGE SAB2700747 diluted at 1:1000
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| NCBI accession no. | NP_037385   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, mouse |
| storage temp. | −20°C |
| technique(s) | immunofluorescence: suitable |
| immunohistochemistry: 1:100-1:1000 | |
| western blot: 500-3000 | |
| UniProt accession no. | Q9UBP4 |
| Application: | Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IHC-P: 1:100-1:1000, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Biochem/physiol Actions: | This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq] |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Recombinant fragment corresponding to a region within amino acids 6 and 343 of DKK3 according to NP_037385 |
| Physical form: | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 - H412 |
| Precautionary statements | P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |