Anti-BCL6 antibody produced in rabbit
SIGMA/SAB2700765 - affinity isolated antibody, buffered aqueous solution
Synonym: BCL5; BCL6; BCL6A; LAZ3
Product Type: Product-on-demand
Immunohistochemistry Immunohistochemical analysis of paraffin-embedded human tonsil, using BCL6 (SAB2700765) antibody (10 μg/ml).
Western Blotting Sample (30 μg of whole cell lysate) A: Jurkat B: K562 C: THP-1 7.5% SDS PAGE SAB2700765 diluted at 1:1000
Western Blotting BCL6 antibody [N2C1], Internal detects BCL6 protein by western blot analysis. A. 30 μg Neuro2A whole cell lysate/extract B. 30 μg GL261 whole cell lysate/extract C. 30 μg C8D30 whole cell lysate/extract D. 30 μg NIH-3T3 whole cell lysate/extract E. 30 μg Raw264.7 whole cell lysate/extract F. 30 μg C2C12 whole cell lysate/extract 7.5% SDS-PAGE BCL6 antibody [N2C1], Internal (SAB2700765) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunoprecipitation BCL6 antibody immunoprecipitates BCL6 protein in IP experiments. IP Sample: Raji whole cell lysate/extract A : 30 μg whole cell lysate/extract of BCL6 protein expressing Raji cells B : Control with 3 μg of pre-immune rabbit IgG C : Immunoprecipitation of BCL6 by 3 μg of BCL6 antibody (SAB2700765) 7.5% SDS-PAGE The immunoprecipitated BCL6 protein was detected by BCL6 antibody (SAB2700765) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.
ChIP Assay Cross-linked ChIP was performed with Raji chromatin extract and 5 μg of either control rabbit IgG or anti-BCL6 antibody. The precipitated DNA was detected by PCR with primer set targeting to PRDM1 or CD20.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| NCBI accession no. | NP_001697   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ChIP: suitable |
| immunohistochemistry: suitable | |
| immunoprecipitation (IP): suitable | |
| western blot: 1:500-1:3,000 | |
| UniProt accession no. | P41182 |
| Application: | Suggested starting dilutions are as follows: IHC-P: 1:100-1:1000, IP: 1:100-1:500, WB: 1:500-1:3000, ChIP assay: Assay-dependent dilution. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Biochem/physiol Actions: | The protein encoded by this gene is a zinc finger transcription factor and contains an N-terminal POZ domain. This protein acts as a sequence-specific repressor of transcription, and has been shown to modulate the transcription of START-dependent IL-4 responses of B cells. This protein can interact with a variety of POZ-containing proteins that function as transcription corepressors. This gene is found to be frequently translocated and hypermutated in diffuse large-cell lymphoma (DLCL), and may be involved in the pathogenesis of DLCL. Alternatively spliced transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq] |
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| Immunogen: | Recombinant fragment corresponding to a region within amino acids 160 and 415 of according to NP_001697 |
| Physical form: | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 - H412 |
| Precautionary statements | P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |