Anti-SLC25A1 antibody produced in rabbit
SIGMA/SAB2700800 - affinity isolated antibody, buffered aqueous solution
Synonym: CTP; SLC20A3; SLC25A1
Product Type: Product-on-demand
Immunofluorescence SLC25A1 antibody [N1C1] detects SLC25A1 protein at mitochondria by immunofluorescent analysis. Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min. Green: SLC25A1 protein stained by SLC25A1 antibody [N1C1] (SAB2700800) diluted at 1:500. Blue: Hoechst 33342 staining.
Western Blotting Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SLC25A1 antibody [N1C1] (SAB2700800) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blotting Rat tissue extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SLC25A1 antibody [N1C1] (SAB2700800) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blotting Mouse tissue extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SLC25A1 antibody [N1C1] (SAB2700800) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| NCBI accession no. | NP_005975   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| technique(s) | immunofluorescence: suitable |
| western blot: 500-3000 | |
| UniProt accession no. | P53007 |
| Application: | Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Biochem/physiol Actions: | The mitochondrial tricarboxylate transporter (also called citrate transport protein, or CTP) is responsible for the movement of citrate across the mitochondrial inner membrane (Kaplan et al., 1993 [PubMed 8514800]).[supplied by OMIM] |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Recombinant fragment corresponding to a region within amino acids 1 and 185 of SLC25A1 according to NP_005975 |
| Physical form: | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 - H412 |
| Precautionary statements | P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |