Anti-RPS3 antibody produced in rabbit
SIGMA/SAB2700840 - affinity isolated antibody, buffered aqueous solution
Synonym: FLJ26283; FLJ27450; MGC87870; RPS3
Product Type: Product-on-demand
Immunohistochemistry Immunohistochemical analysis of paraffin-embedded SAS xenograft, using RPS3(SAB2700840) antibody at 1:500 dilution.
Immunohistochemistry RPS3 antibody detects RPS3 protein at cytoplasm in mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. RPS3 antibody (SAB2700840) diluted at 1:500.
Immunohistochemistry RPS3 antibody detects RPS3 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. RPS3 antibody (SAB2700840) diluted at 1:500.
Immunofluorescence Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using RPS3 (SAB2700840) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled (Red)
Western Blotting RPS3 antibody detects RPS3 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RPS3 antibody (SAB2700840) diluted at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| NCBI accession no. | NP_000996   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| technique(s) | immunofluorescence: suitable |
| immunohistochemistry: suitable | |
| immunoprecipitation (IP): suitable | |
| western blot: 500-3000 | |
| UniProt accession no. | P23396 |
| Application: | Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IHC-P: 1:100-1:1000, IP: Assay-dependent dilution, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Biochem/physiol Actions: | Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit, where it forms a part of the domain where translation is initiated. Studies of the mouse and rat proteins have demonstrated that the protein has an extra ribosomal role as an endonuclease involved in the repair of UV-induced DNA damage. Higher levels of expression of this gene in colon adenocarcinomas and adenomatous polyps compared to adjacent normal colonic mucosa have been observed. This gene is co-transcribed with the small nucleolar RNA genes U15A and U15B, which are located in its first and fifth introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq]. RPS3 (ribosomal protein S3) has endonuclease activity for DNA repair mechanism. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | RPS3 (ribosomal protein S3) codes for a translational protein which belongs to the ribosomal small subunit protein. It is localized to the surface of 40s ribosomal subunit. The RPS3 gene is mapped to human chromosome 11q13.4. The protein belongs to the S3P family of ribosomal proteins and appears to be located in both the cytoplasm and nucleus but not in the nucleolus. |
| Immunogen: | Recombinant fragment corresponding to a region within amino acids 1 and 243 of RPS3 according to NP_000996 |
| Physical form: | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 - H412 |
| Precautionary statements | P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |