Anti-AKR1C1 antibody produced in rabbit
SIGMA/SAB2700882 - affinity isolated antibody, buffered aqueous solution
Synonym: 2-ALPHA-HSD; 20-ALPHA-HSD; AKR1C1; C9
Product Type: Product-on-demand
Immunohistochemistry Immunohistochemical analysis of paraffin-embedded MDA-MB-468 xenograft, using AKR1C1(SAB2700882) antibody at 1:500 dilution.
Immunofluorescence Immunofluorescence analysis of methanol-fixed A431, using AKR1C1(SAB2700882) antibody at 1:200 dilution.
Western Blotting Sample (30 μg of whole cell lysate) A: A549 10% SDS PAGE SAB2700882 diluted at 1:1000
Western Blotting Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKR1C1 antibody (SAB2700882) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blotting AKR1C1 antibody detects AKR1C1 protein by western blot analysis. A. 50 μg mouse liver lysate/extract 10% SDS-PAGE AKR1C1 antibody (SAB2700882) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| NCBI accession no. | NP_001344   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| immunohistochemistry: suitable | |
| western blot: 500-3000 | |
| UniProt accession no. | Q04828 |
| Application: | Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IHC: Assay-dependent dilution, IHC-P: 1:100-1:1000, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Biochem/physiol Actions: | This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reaction of progesterone to the inactive form 20-alpha-hydroxy-progeste |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Recombinant fragment corresponding to a region within amino acids 22 and 248 of AKR1C1 according to NP_001344 |
| Physical form: | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 - H412 |
| Precautionary statements | P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |