Anti-CD2AP antibody produced in rabbit
SIGMA/SAB2700911 - affinity isolated antibody, buffered aqueous solution
Synonym: CD2AP; CMS; DKFZp586H0519
Product Type: Product-on-demand
Immunohistochemistry Immunohistochemical analysis of paraffin-embedded FaDu xenograft, using CD2-associated protein(SAB2700911) antibody at 1:500 dilution.
Immunohistochemistry CD2AP antibody detects CD2AP protein at cytosol on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. CD2AP antibody (SAB2700911) dilution: 1:500.
Immunofluorescence Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using CD2AP (SAB2700911) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled (Red).
Western Blotting Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with CD2AP antibody (SAB2700911) diluted at 1:1000 and competitor′s antibody (sc-9137) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blotting CD2AP antibody detects CD2AP protein by western blot analysis. A. 30 μg Rat2 whole cell lysate/extract 7.5% SDS-PAGE CD2AP antibody (SAB2700911) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blotting Sample (30 μg of whole cell lysate) A: A431 7.5% SDS PAGE SAB2700911 diluted at 1:1000
Western Blotting CD2AP antibody detects CD2AP protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract D. 30 μg HepG2 whole cell lysate/extract E. 30 μg A375 whole cell lysate/extract 7.5% SDS-PAGE CD2AP antibody (SAB2700911) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| NCBI accession no. | NP_036252   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| indirect immunofluorescence: suitable | |
| western blot: 500-3000 | |
| UniProt accession no. | Q9Y5K6 |
| Application: | Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IHC-P: 1:100-1:1000, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Biochem/physiol Actions: | This gene encodes a scaffolding molecule that regulates the actin cytoskeleton. The protein directly interacts with filamentous actin and a variety of cell membrane proteins through multiple actin binding sites, SH3 domains, and a proline-rich region containing binding sites for SH3 domains. The cytoplasmic protein localizes to membrane ruffles, lipid rafts, and the leading edges of cells. It is implicated in dynamic actin remodeling and membrane trafficking that occurs during receptor endocytosis and cytokinesis. Haploinsufficiency of this gene is implicated in susceptibility to glomerular disease. [provided by RefSeq] |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Recombinant fragment corresponding to a region within amino acids 1 and 281 of CD2AP according to NP_036252 |
| Physical form: | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |