Anti-CDT1 antibody produced in rabbit
SIGMA/SAB2701049 - affinity isolated antibody, buffered aqueous solution
Synonym: CDT1; DUP; RIS2
Product Type: Product-on-demand
Immunohistochemistry Immunohistochemical analysis of paraffin-embedded ES2 xenograft, using CDT1(SAB2701049) antibody at 1:500 dilution.
Immunofluorescence CDT1 antibody detects CDT1 protein at nucleus by immunofluorescent analysis. Sample: SK-N-AS cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CDT1 protein stained by CDT1 antibody (SAB2701049) diluted at 1:500. Blue: Hoechst 33342 staining.
Western Blotting CDT1 antibody detects CDT1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CDT1 antibody (SAB2701049) diluted at a dilution of 1:1000.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| NCBI accession no. | AAH08676   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| indirect immunofluorescence: suitable | |
| western blot: 500-3000 | |
| UniProt accession no. | Q9H211 |
| Application: | Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IHC-P: 1:100-1:1000, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Biochem/physiol Actions: | Chromatin licensing and DNA replication factor 1/Cdc10-dependent transcript 1 (CDT1) is involved in minichromosome maintenance (MCM) loading at the G1 phase of the cell cycle. It plays a key role in origin licensing in eukaryotic cells. It is a DNA replication protein that stimulates the production of pre-replicative complexes and replication in the next cell cycle by accumulating at the end of the cell cycle. The CDT1 gene mutation can cause Meier–Gorlin syndrome, a type of primordial dwarfism. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Chromatin licensing and DNA replication factor 1/Cdc10-dependent transcript 1 (CDT1), a G1-specific cell-cycle factor, belongs to the pre-replication complex (pre-RC). The CDT1 gene is located on the human chromosome 16q24.3. |
| Immunogen: | Synthetic peptide containing a sequence corresponding to a region within amino acids 448 and 490 (Q9H211) of CDT1 according to AAH08676 |
| Physical form: | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 - H412 |
| Precautionary statements | P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |