Anti-GNB1 antibody produced in rabbit
SIGMA/SAB2701168 - affinity isolated antibody, buffered aqueous solution
Synonym: GNB1; guanine nucleotide binding protein (G protein), beta polypeptide 1
Product Type: Product-on-demand
Immunohistochemistry Immunohistochemical analysis of paraffin-embedded human lung papillory adenocarcinoma, using GNB1(SAB2701168) antibody at 1:500 dilution.
Immunohistochemistry GNB1 antibody detects GNB1 protein at cell membrane in mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. GNB1 antibody (SAB2701168) diluted at 1:1000.
Immunohistochemistry GNB1 antibody detects GNB1 protein at cell membrane in rat testis by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. GNB1 antibody (SAB2701168) diluted at 1:1000.
Immunofluorescence GNB1 antibody detects GNB1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GNB1 protein stained by GNB1 antibody (SAB2701168) diluted at 1:500. Red: FIS1, a mitochondria marker, stained by FIS1 antibody. Blue: Hoechst 33342 staining.
Western Blotting Sample (30 μg of whole cell lysate) A: JurKat 10% SDS PAGE SAB2701168 diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blotting Sample (50 μg of whole cell lysate) A: mouse brain 10% SDS PAGE SAB2701168 diluted at 1:10,000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunoprecipitation Immunoprecipitation of GNB1 protein from 293T whole cell extracts using 5 μg of GNB1 antibody (SAB2701168). Western blot analysis was performed using GNB1 antibody (SAB2701168). EasyBlot anti-Rabbit IgG was used as a secondary reagent.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| NCBI accession no. | AAH04186   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| immunoprecipitation (IP): suitable | |
| indirect immunofluorescence: suitable | |
| western blot: 5000-20000 | |
| UniProt accession no. | P62873 |
| Application: | Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IHC-P: 1:100-1:1000, IP: 1:100-1:500, WB: 1:5000-1:20000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Biochem/physiol Actions: | Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. This gene uses alternative polyadenylation signals. [provided by RefSeq] |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Recombinant protein fragment contain a sequence corresponding to a region within amino acids 67 and 271 of GNB1 according to AAH04186 |
| Physical form: | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 - H412 |
| Precautionary statements | P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |