Anti-PHF10 antibody produced in rabbit
SIGMA/SAB2701275 - affinity isolated antibody, buffered aqueous solution
Synonym: BAF45A; FLJ10975; MGC111009; PHF10
Product Type: Product-on-demand
Immunohistochemistry PHF10 antibody detects PHF10 protein at nucleus in mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. PHF10 antibody (SAB2701275) diluted at 1:500.
Immunohistochemistry Immunohistochemical analysis of paraffin-embedded HeLa xenograft, using PHF10(SAB2701275) antibody at 1:500 dilution.
Immunohistochemistry PHF10 antibody detects PHF10 protein at nucleus in rat lung by immunohistochemical analysis. Sample: Paraffin-embedded rat lung. PHF10 antibody (SAB2701275) diluted at 1:500.
Immunofluorescence PHF10 antibody detects PHF10 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PHF10 protein stained by PHF10 antibody (SAB2701275) diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:100. Blue: Hoechst 33342 staining.
Western Blotting PHF10 antibody detects PHF10 protein by western blot analysis. A.50 μg mouse colon lysate/extract 10% SDS-PAGE PHF10 antibody (SAB2701275) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blotting HeLa whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PHF10 antibody (SAB2701275) diluted at 1:1000.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| NCBI accession no. | AAH20954   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| indirect immunofluorescence: suitable | |
| western blot: 500-3000 | |
| UniProt accession no. | Q8WUB8 |
| Application: | Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IHC-P: 1:100-1:1000, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Biochem/physiol Actions: | This gene contains a predicted ORF that encodes a protein with two zinc finger domains. The function of the encoded protein is not known. Sequence analysis suggests that multiple alternatively spliced transcript variants are derived from this gene but the full-length nature of only two of them is known. These two splice variants encode different isoforms. A pseudogene for this gene is located on Xq28. [provided by RefSeq] |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Recombinant fragment corresponding to a region within amino acids 243 and 459 of PHF10 according to AAH20954 |
| Physical form: | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |