Anti-SMC6 antibody produced in rabbit
SIGMA/SAB2701298 - affinity isolated antibody, buffered aqueous solution
Synonym: FLJ22116; FLJ35534; SMC6; SMC6L1
Product Type: Product-on-demand
Immunohistochemistry Immunohistochemical analysis of paraffin-embedded ES2 xenograft, using SMC6L1(SAB2701298) antibody at 1:500 dilution.
Immunofluorescence SMC6L1 antibody [N3C2], Internal detects SMC6 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SMC6 protein stained by SMC6L1 antibody [N3C2], Internal (SAB2701298) diluted at 1:500. Blue: Hoechst 33342 staining.
Western Blotting Sample (30 μg of whole cell lysate) A: NT2D1 7.5% SDS PAGE SAB2701298 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| NCBI accession no. | AAH39828   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| indirect immunofluorescence: suitable | |
| western blot: 500-3000 | |
| UniProt accession no. | Q96SB8 |
| Application: | Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IHC-P: 1:100-1:1000, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Biochem/physiol Actions: | Core component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Recombinant fragment corresponding to a region within amino acids 526 and 776 of SMC6L1 according to AAH39828 |
| Physical form: | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |