Anti-RAB35 antibody produced in rabbit
SIGMA/SAB2701465 - affinity isolated antibody, buffered aqueous solution
Synonym: H-ray; RAB1C; RAB35; RAY
Product Type: Product-on-demand
Immunohistochemistry Immunohistochemical analysis of paraffin-embedded human breast cancer, using RAB35(SAB2701465) antibody at 1:250 dilution.
Western Blotting Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with RAB35 antibody [N1C3] (SAB2701465) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Western Blotting Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB35 antibody [N1C3] (SAB2701465) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blotting Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with RAB35 antibody [N1C3] (SAB2701465) diluted at 1:1000.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| NCBI accession no. | AAH15931   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, mouse, hamster |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry: suitable |
| indirect immunofluorescence: suitable | |
| western blot: 500-3000 | |
| UniProt accession no. | Q15286 |
| Application: | Suggested starting dilutions are as follows: ICC/IF: Assay-dependent dilution, IHC-P: 1:100-1:1000, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Recombinant fragment corresponding to a region within amino acids 1 and 201 of RAB35 according to AAH15931 |
| Physical form: | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 - H412 |
| Precautionary statements | P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |