Anti-PCNA antibody produced in rabbit
SIGMA/SAB2701819 - affinity isolated antibody, buffered aqueous solution
Synonym: cb16; fb36g03; pcna; wu:fa28e03
Product Type: Product-on-demand
Immunohistochemistry Immunohistochemical analysis of paraffin-embedded zebrafish kidney, using pcna (SAB2701819) antibody at 1:300 dilution.
Immunohistochemistry Pcna antibody detects Pcna protein on zebrafish by whole mount immunohistochemical analysis. Sample: Paraformaldehyde-fixed 2 day-post-fertilization zebrafish embryo. Pcna antibody (SAB2701819) dilution: 1:50.
Immunohistochemistry Pcna antibody detects Pcna protein on whole mount zebrafish by immunohistochemical analysis. Sample: 2 day-post-fertilization zebrafish embryo. Pcna antibody (SAB2701819) dilution: 1:100.
Immunohistochemistry Pcna antibody detects Pcna protein on zebrafish by whole mount immunohistochemical analysis. Sample: Paraformaldehyde-fixed 2 day-post-fertilization zebrafish embryo. Pcna antibody (SAB2701819) dilution: 1:50.
Immunohistochemistry Pcna antibody detects Pcna protein on Medaka by whole mount immunohistochemical analysis. Sample: 7 days-post-fertilization medaka embryo. Pcna antibody (SAB2701819) dilution: 1:100.
Western Blotting Various tissue extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Pcna antibody (SAB2701819) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blotting PCNA antibody detects PCNA protein by western blot analysis. Zebrafish tissue extracts (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with PCNA antibody (SAB2701819) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| NCBI accession no. | NP_571479   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | zebrafish, (Japanese Medaka) |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| western blot: 500-3000 |
| Application: | Suggested starting dilutions are as follows: IHC-P: 1:100-1:1000, WB: 1:500-1:3000, IHC-Wm: 1:50-1:500, Immunostaining: Assay-dependent dilution. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. Anti-PCNA antibody has been used in the detection of proliferating cells by immunofluorescence. |
| Biochem/physiol Actions: | This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase′s processibility during elongation of the leading strand. Proliferating cell nuclear antigen (PCNA) controls the production of the leading and lagging strands, which are required for the duplication of DNA. It acts as a cell cycle regulatory protein. This protein regulates apoptosis. PCNA is also involved in non-replicative DNA synthesis events. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Proliferating cell nuclear antigen (PCNA) is a ring-shaped homotrimer protein, that is located at the center of the faithful duplication of eukaryotic genomes. Since it encircles the DNA, PCNA is also known as a sliding clamp. This gene is mapped to human chromosome 20p12.3. |
| Immunogen: | Recombinant protein containing a sequence corresponding to a region within amino acids 31 and 222 of pcna according to NP_571479 |
| Physical form: | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 - H412 |
| Precautionary statements | P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |