Anti-HMGA2 antibody produced in rabbit
SIGMA/SAB2701959
Synonym: HMGI-C; HMGIC; LIPO; STQTL9
Product Type: Product-on-demand
Immunohistochemistry HMGA2 antibody - ChIP grade detects HMGA2 protein at nucleus in human HuCCT1 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded human HuCCT1 xenograft . HMGA2 antibody - ChIP grade (SAB2701959) diluted at 1:250.
Immunohistochemistry HMGA2 antibody detects HMGA2 protein at nucleus in rat duodenum by immunohistochemical analysis. Sample: Paraffin-embedded rat duodenum. HMGA2 antibody (SAB2701959) diluted at 1:500.
Immunohistochemistry HMGA2 antibody detects HMGA2 protein at nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. HMGA2 antibody (SAB2701959) diluted at 1:500.
Immunohistochemistry Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using HMGA2(SAB2701959) antibody at 1:500 dilution.
Immunohistochemistry Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using HMGA2(SAB2701959) antibody at 1:500 dilution.
Immunofluorescence HMGA2 antibody detects HMGA2 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HMGA2 protein stained by HMGA2 antibody (SAB2701959) diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 μm.
Western Blotting HMGA2 antibody detects HMGA2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with HMGA2 antibody (SAB2701959) diluted at a dilution of 1:10,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blotting Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with HMGA2 antibody - ChIP grade (SAB2701959) diluted at 1:50,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blotting HMGA2 antibody detects HMGA2 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with HMGA2 antibody (SAB2701959) at a dilution of 1:10,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunoprecipitation Immunoprecipitation of HMGA2 protein from HepG2 nuclear extracts using 5 μg of HMGA2 antibody (SAB2701959). Western blot analysis was performed using HMGA2 antibody (SAB2701959). EasyBlot anti-Rabbit IgG was used as a secondary reagent.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.7 mg/mL |
| conjugate | unconjugated |
| form | liquid |
| mol wt | 12 kDa |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ChIP: suitable |
| immunofluorescence: suitable | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| immunoprecipitation (IP): suitable | |
| western blot: 5000-50000 | |
| UniProt accession no. | P52926  |
| Application: | Anti-HMGA2 antibody produced in rabbit has been used in immunohistochemistry (1:250), (1:100), (1:4000). |
| Biochem/physiol Actions: | High mobility group A2 (HMGA2) protein is involved in regulating the activity of various genes by binding to the adenine/thymine (AT)-rich sequences present in the minor groove of B‑form DNA. This protein plays a role in many biological processes such as the DNA damage repair process, senescence, apoptosis, and cell cycle process. HMGA2 protein also plays a role in epithelial-mesenchymal transition and telomere restoration. Overexpression of HMG2A gene is observed in metastatic lung adenocarcinoma. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | High mobility group A2 (HMGA2) protein is a non-histone architectural transcription factor. This protein belongs to the high-mobility group adenine/thymine (AT)-hook (HMGA) family. HMGA2 is expressed in the mesenchyme prior differentiation and tumors of epithelial origin. The HMGA2 gene is located on the human chromosome at 12q14.3. |
| Immunogen: | Recombinant fragment corresponding to a region within amino acids 1 and 109 of HMGA2 (Uniprot ID#P52926) |
| Physical form: | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 - H412 |
| Precautionary statements | P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |