Anti-HSPA8 antibody produced in rabbit
SIGMA/SAB2701964
Synonym: HSP73; HSPA10; LAP1; NIP71
Product Type: Product-on-demand
Immunohistochemistry HSC70 antibody detects HSC70 protein at nucleolus on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. HSC70 antibody (SAB2701964) dilution: 1:500.
Immunohistochemistry Immunohistochemical analysis of paraffin-embedded U87 xenograft, using HSC70 (SAB2701964) antibody at 1:100 dilution.
Immunohistochemistry HSC70 antibody detects HSC70 protein at nucleolus on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. HSC70 antibody (SAB2701964) dilution: 1:500.
Immunofluorescence Immunofluorescence analysis of methanol-fixed HeLa, using HSC70(SAB2701964) antibody at 1:200 dilution.
Western Blotting Sample (50 μg of whole cell lysate) A: Mouse brain 7.5% SDS PAGE SAB2701964 diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blotting Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSC70 antibody (SAB2701964) diluted at 1:10,000.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 10 mg/mL |
| conjugate | unconjugated |
| form | liquid |
| mol wt | 71 kDa |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunofluorescence: suitable | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| western blot: 1000-20000 | |
| UniProt accession no. | P11142  |
| Application: | Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IHC-P: 1:100-1:1000, WB: 1:1000-1:20000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Biochem/physiol Actions: | The product encoded by this gene belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. This gene encodes a heat-shock cognate protein. This protein binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Two alternatively spliced variants have been characterized to date. [provided by RefSeq] |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Recombinant protein fragment contain a sequence corresponding to a region within amino acids 470 and 635 (P11142) of Hsc70 |
| Physical form: | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 - H412 |
| Precautionary statements | P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |