Anti-SOX2 antibody produced in rabbit
SIGMA/SAB2701974
Synonym: ANOP3; ANOP3; MCOPS3
Product Type: Product-on-demand
Immunohistochemistry SOX2 antibody [N1C3] detects SOX2 protein at nucleus on rat brain stem by immunohistochemical analysis. Sample: Paraffin-embedded rat brain stem. SOX2 antibody [N1C3] (SAB2701974) dilution: 1:500.
Immunohistochemistry SOX2 antibody [N1C3] detects SOX2 protein at nucleus by immunohistochemical analysis. Sample: Frozen sectioned E13.5 rat brain. Green: SOX2 protein stained by SOX2 antibody [N1C3] (SAB2701974) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody.. Blue: Fluoroshield with DAPI.
Immunohistochemistry SOX2 antibody [N1C3] detects SOX2 protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse hippocampus. Green: SOX2 protein stained by SOX2 antibody [N1C3] (SAB2701974) diluted at 1:250. Blue: Fluoroshield with DAPI.
Immunohistochemistry SOX2 antibody [N1C3] detects SOX2 protein at nucleus in mouse esophagus by immunohistochemical analysis. Sample: Paraffin-embedded mouse esophagus. SOX2 antibody [N1C3] (SAB2701974) diluted at 1:500.
Immunohistochemistry Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using SOX2(SAB2701974) antibody at 1:100 dilution.
Immunohistochemistry SOX2 antibody [N1C3] detects SOX2 protein at nucleus in human esophageal carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human esophageal carcinoma. SOX2 antibody [N1C3] (SAB2701974) diluted at 1:500.
Immunohistochemistry SOX2 antibody [N1C3] detects SOX2 protein at nucleus by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: SOX2 protein stained by SOX2 antibody [N1C3] (SAB2701974) diluted at 1:250. Red: Protein kinase C alpha staining. Blue: Fluoroshield with DAPI.
Immunohistochemistry SOX2 antibody [N1C3] detects SOX2 protein at nucleus in mouse fetal brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fetal brain. Green: SOX2 antibody [N1C3] (SAB2701974) diluted at 1:200. Blue: Nuclear staining with Hoechst 33342.
Immunohistochemistry SOX2 antibody [N1C3] detects SOX2 protein at nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. SOX2 antibody [N1C3] (SAB2701974) diluted at 1:500.
Immunohistochemistry SOX2 antibody [N1C3] detects SOX2 protein at nucleus in human cervical carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. SOX2 antibody [N1C3] (SAB2701974) diluted at 1:500.
Immunofluorescence Immunofluorescence analysis of paraformaldehyde-fixed mouse ESC, using SOX2(SAB2701974) antibody at 1:80 dilution.
Immunofluorescence SOX2 antibody [N1C3] detects SOX2 protein at nucleus by immunofluorescent analysis. Sample: NT2/D1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SOX2 protein stained by SOX2 antibody [N1C3] (SAB2701974) diluted at 1:1000. Red: Phalloidin, a cytoskeleton marker, diluted at 1:50.
Immunofluorescence Immunofluorescence analysis of paraformaldehyde-fixed Human ESC, using SOX2(SAB2701974) antibody at 1:160 dilution.
Western Blotting SOX2 antibody detects SOX2 protein by western blot analysis. Mouse ESC lysate (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with SOX2 antibody (SAB2701974) at a dilution of 1:2500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blotting Non-transfected (–) and transfected (+) NT2D1 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SOX2 antibody [N1C3] (SAB2701974) diluted at 1:15000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blotting Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SOX2 antibody [N1C3] (SAB2701974) diluted at 1:10,000.
Western Blotting SOX2 antibody detects SOX2 protein by Western blot analysis. Whole cell extracts (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SOX2 antibody (SAB2701974) at a dilution of 1:2500.
Immunoprecipitation Immunoprecipitation of SOX2 protein from NT2D1 whole cell extracts using 5 μg of SOX2 antibody [N1C3] (SAB2701974). Western blot analysis was performed using SOX2 antibody [N1C3] (SAB2701974). EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Immunoprecipitation Immunoprecipitation of SOX2 protein from NT2D1 whole cell extracts using 5 μg of SOX2 antibody [N1C3] (SAB2701974) or SOX2 antibody. Western blot analysis was performed using SOX2 antibody [N1C3] (SAB2701974). EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Flow Cytometry Flow cytometry on human embryonic stem cells, staining with SOX2 (SAB2701974)antibody at 1:100 dilution(purple) or rabbit IgG (black).
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| form | liquid |
| mol wt | 34 kDa |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| technique(s) | flow cytometry: 50-200 |
| immunocytochemistry: suitable | |
| immunofluorescence: suitable | |
| immunohistochemistry: suitable | |
| immunoprecipitation (IP): suitable | |
| western blot: 500-20000 | |
| UniProt accession no. | P48431  |
| Application: | Suggested starting dilutions are as follows: FACS: 1:50-1:200, ICC/IF: 1:100-1:1000, IHC-Fr: 1:100-1:1000, IHC-P: 1:100-1:1000, IP: 1:100-1:500, WB: 1:500-1:20000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Biochem/physiol Actions: | SOX2 (sex-determining region Y-box 2) down-regulation reduces the stem cell count, that affects the breast cancer cells in initiating tumor progression. It participates in chemoresistance and regular lung cancer therapies. In mouse, SOX2 plays an important role in branching morphogenesis and regulating lung epithelial cell differentiation. |
| Biochem/physiol Actions: | This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq] |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | SOX2 (sex-determining region Y-box 2) is a transcription factor that is made of 317 amino acids. It has a HMG (high mobility group box) domain and is a critical transcription regulator of normal stem cells. This gene is located on human chromosome 3q26.3. |
| Immunogen: | Recombinant fragment corresponding to a region within amino acids 1 and 291 of SOX2 (Uniprot ID#P48431) |
| Physical form: | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 - H412 |
| Precautionary statements | P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |