Anti-DBH antibody produced in rabbit
SIGMA/SAB2701977
Synonym: DBM
Product Type: Product-on-demand
Immunohistochemistry Dopamine beta hydroxylase antibody detects DBH protein at cytosol on RT2 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded RT2 xenograft. Dopamine beta hydroxylase antibody (SAB2701977) dilution: 1:500.
Immunohistochemistry Dopamine beta hydroxylase antibody detects DBH protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. Dopamine beta hydroxylase antibody (SAB2701977) dilution: 1:500.
Immunohistochemistry Dopamine beta hydroxylase antibody detects DBH protein at cytosol on mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Dopamine beta hydroxylase antibody (SAB2701977) dilution: 1:500.
Western Blotting Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Dopamine beta hydroxylase antibody (SAB2701977) diluted at 1:2000 and competitor′s antibody ( #8586S) diluted by 1:200. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| form | liquid |
| mol wt | 69 kDa |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:1,000 |
| western blot: 1:500-1:3,000 | |
| UniProt accession no. | P09172  |
| Application: | Anti-DBH antibody produced in rabbit has been used in immunohistochemistry (1:200) (1:1000). |
| Biochem/physiol Actions: | Dopamine-β-hydroxylase (DBH) protein plays a role in the conversion of dopamine to noradrenaline. This protein is implicated in various disorders such as Huntington′s chorea, hypertension, Parkinson′s, and smoking disorders. DBH protein is also implicated in heart failure and mental disorders like depression, Tourette syndrome, and schizophrenia. Lower levels of DHB plasma are seen in patients with bipolar disorders and unipolar psychotic depression. Higher levels of DBH plasma are observed in patients with severe depression. |
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| General description: | Dopamine-β-hydroxylase (DBH) is found in the nuclei of noradrenergic and dopaminergic neurons. The DBH gene is mapped on the human chromosome at 9q34.2. |
| Immunogen: | Recombinant fragment corresponding to a region within amino acids 8 and 320 of Dopamine beta Hydroxylase (Uniprot ID#P09172) |
| Physical form: | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 - H412 |
| Precautionary statements | P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |