Anti-TRIM28 antibody produced in rabbit
SIGMA/SAB2701980
Synonym: KAP1; RNF96; TF1B; TIF1B
Product Type: Product-on-demand
Immunohistochemistry Immunohistochemical analysis of paraffin-embedded human breast cancer, using KAP1 (SAB2701980) antibody at 1:500 dilution.
Immunofluorescence Immunofluorescence analysis of paraformaldehyde-fixed A431, using KAP1 (SAB2701980) antibody at 1:200 dilution.
Immunofluorescence KAP1 antibody [N3C2], Internal detects KAP1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: KAP1 protein stained by KAP1 antibody [N3C2], Internal (SAB2701980) diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody
Western Blotting Sample (30 μg of whole cell lysate) A: H1299 B: HeLa 7.5% SDS PAGE SAB2701980 diluted at 1:10,000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blotting Sample (30 μg of whole cell lysate) A: NIH-3T3 7.5% SDS PAGE SAB2701980 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunoprecipitation KAP1 antibody immunoprecipitates KAP1 protein in IP experiments. IP Sample: 1000 μg HeLa whole cell lysate/extract A. 50 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of KAP1 protein by 2 μg of KAP1 antibody (SAB2701980) 7.5% SDS-PAGE The immunoprecipitated KAP1 protein was detected by KAP1 antibody (SAB2701980) diluted at 1:1000. EasyBlot anti-Rabbit IgG was used as a secondary reagent.
ChIP Assay ChIP assay followed by QPCR on a known KAP1-binding region within the ZNF180 3’UTR (Mol Cell Biol, 2011, Lyengar et al)
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1.01 mg/mL |
| conjugate | unconjugated |
| form | liquid |
| mol wt | 89 kDa |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ChIP: suitable |
| immunocytochemistry: suitable | |
| immunofluorescence: suitable | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| immunoprecipitation (IP): suitable | |
| western blot: 1000-10000 | |
| UniProt accession no. | Q13263  |
| Application: | Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IHC-P: 1:100-1:1000, IP: 1:500-1:1000, WB: 1:1000-1:10000, ChIP assay: Assay-dependent dilution. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Biochem/physiol Actions: | The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. [provided by RefSeq] |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Recombinant fragment corresponding to a region within amino acids 361 and 613 of KAP1 (Uniprot ID#Q13263) |
| Physical form: | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 - H412 |
| Precautionary statements | P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |