Anti-ABCB1 antibody produced in rabbit
SIGMA/SAB2702025
Synonym: GP170; MDR1; P-GP; PGY1
Product Type: Product-on-demand
Western Blotting Boiled and unboiled HepG2 whole cell and membrane extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with P Glycoprotein antibody (SAB2702025) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.4 mg/mL |
| conjugate | unconjugated |
| form | liquid |
| mol wt | 142 kDa |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1:500-1:3000 |
| UniProt accession no. | P08183  |
| Application: | Suggested starting dilutions are as follows: WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Biochem/physiol Actions: | ABCB1 (ATP-binding cassette, subfamily B 1 gene) or P-glycoprotein 170 (P-gp) participates in the development of tumors, especially in the carcinogenesis of colorectal. The encoded P-gp guards the body from environmental toxins and xenobiotics. The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. [provided by RefSeq] |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | ABCB1 (ATP-binding cassette, subfamily B 1 gene) is also known as multidrug resistance 1 (MDR1) gene. MDR1 codes for P-glycoprotein 170 (P-gp), that is expressed at high levels in various cells and epithelial surfaces, including the epithelium of GI (gastrointestinal) tract. ABCB1 gene is located on human chromosome 7q. |
| Immunogen: | Synthetic peptide corresponding to a region within amino acids 1 and 45 of P Glycoprotein |
| Physical form: | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 - H412 |
| Precautionary statements | P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |