Anti-POU3F2 antibody produced in rabbit
SIGMA/SAB2702086
Synonym: OCT-7; OTF7; POUF3; brn-2
Product Type: Product-on-demand
Immunohistochemistry Immunohistochemical analysis of paraffin-embedded TOV-21G xenograft, using Brn2(SAB2702086) antibody at 1:500 dilution.
Immunohistochemistry Brn2 antibody detects Brn2 protein expression at nucleus by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: Brn2 protein stained by Brn2 antibody (SAB2702086) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody. Blue: Fluoroshield with DAPI.
Immunohistochemistry Brn2 antibody detects Brn2 protein at nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Brn2 antibody (SAB2702086) diluted at 1:500.
Immunohistochemistry Brn2 antibody detects Brn2 protein at nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Brn2 antibody (SAB2702086) diluted at 1:500.
Immunofluorescence Brn2 antibody detects Brn2 protein at nucleus by immunofluorescent analysis. Sample: SK-N-AS cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Brn2 protein stained by Brn2 antibody (SAB2702086) diluted at 1:1000. Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody . Scale bar = 10 μm.
Western Blotting Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Brn2 antibody (SAB2702086) diluted at 1:1000.
Immunoprecipitation Brn2 antibody immunoprecipitates Brn2 protein in IP experiments. IP Sample: 1000 μg 293T whole cell lysate/extract A. 50 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of Brn2 protein by 2 μg of Brn2 antibody (SAB2702086) 10% SDS-PAGE The immunoprecipitated Brn2 protein was detected by Brn2 antibody (SAB2702086) diluted at 1:1000. EasyBlot anti-Rabbit IgG was used as a secondary reagent.
ChIP Assay Brn2 antibody immunoprecipitates Brn2 protein-DNA in ChIP experiments. ChIP Sample: 293T whole cell lysate/extract A. 5 μg preimmune rabbit IgG B. 5 μg of Brn2 antibody (SAB2702086) The precipitated DNA was detected by PCR with primer set targeting to MITF promoter.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| form | liquid |
| mol wt | 47 kDa |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ChIP: suitable |
| immunocytochemistry: suitable | |
| immunofluorescence: suitable | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| immunoprecipitation (IP): suitable | |
| western blot: 500-3000 | |
| UniProt accession no. | P20265  |
| Application: | Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IHC: Assay-dependent dilution, IHC-Fr: 1:100-1:1000, IHC-P: 1:100-1:1000, IP: 1:100-1:500, WB: 1:500-1:3000, ChIP assay: Assay-dependent dilution. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Biochem/physiol Actions: | POU3F2 belongs to a large family of transcription factors that bind to the octameric DNA sequence ATGCAAAT. Most of these proteins share a highly homologous region, referred to as the POU domain, that occurs in several mammalian transcription factors, including the octamer-binding proteins Oct1 (POU2F1; MIM 164175) and Oct2 (POU2F2; MIM 164176) and the pituitary protein Pit1 (PIT1; MIM 173110). Class III POU genes are expressed predominantly in the central nervous system (CNS). It is likely that CNS-specific transcription factors such as these play an important role in mammalian neurogenesis by regulating their diverse patterns of gene expression (Schreiber et al., 1993 [PubMed 8441633]; Atanasoski et al., 1995 [PubMed 7601453]).[supplied by OMIM] |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Recombinant fragment corresponding to a region within amino acids 248 and 443 of Brn2 (Uniprot ID#P20265) |
| Physical form: | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 - H412 |
| Precautionary statements | P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |