Anti-KDM6A antibody produced in rabbit
SIGMA/SAB2702123
Synonym: KABUK2; UTX; bA386N14.2
Product Type: Product-on-demand
Immunohistochemistry Immunohistochemical analysis of paraffin-embedded H441 xenograft, using KDM6A(SAB2702123) antibody at 1:250 dilution.
Immunohistochemistry KDM6A antibody [N2C1], Internal detects KDM6A protein at nucleus on mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. KDM6A antibody [N2C1], Internal (SAB2702123) dilution: 1:1000.
Immunofluorescence Immunofluorescence analysis of paraformaldehyde-fixed MCF-7, using KDM6A(SAB2702123) antibody at 1:500 dilution.
Western Blotting Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with KDM6A antibody [N2C1], Internal (SAB2702123) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunoprecipitation KDM6A antibody [N2C1], Internal immunoprecipitates KDM6A protein in IP experiments. IP samples: SK-N-SH whole cell extract A. 30 μg SK-N-SH whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of KDM6A protein by 4 μg KDM6A antibody [N2C1], Internal (SAB2702123) 5 % SDS-PAGE The immunoprecipitated KDM6A protein was detected by KDM6A antibody [N2C1], Internal (SAB2702123) diluted at 1:500. [EasyBlot anti-Rabbit IgG was used as a secondary reagent].
ChIP Assay ChIP was performed with HeLa chromatin extract and 5 μg of either normal rabbit IgG or anti-KDM6A antibody. The precipitated DNA was detected by PCR with primer set targeting to RBL1 promoter.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.9 mg/mL |
| conjugate | unconjugated |
| form | liquid |
| mol wt | 154 kDa |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ChIP: suitable |
| immunocytochemistry: suitable | |
| immunofluorescence: suitable | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| immunoprecipitation (IP): suitable | |
| western blot: 500-3000 | |
| UniProt accession no. | O15550  |
| Application: | Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IHC-P: 1:100-1:1000, IP: 1:100-1:500, WB: 1:500-1:3000, ChIP assay: Assay-dependent dilution. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Biochem/physiol Actions: | This gene is located on the X chromosome and is the corresponding locus to a Y-linked gene which encodes a tetratricopeptide repeat (TPR) protein. The encoded protein of this gene contains a JmjC-domain and catalyzes the demethylation of tri/dimethylated histone H3. [provided by RefSeq] |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Recombinant fragment corresponding to a region within amino acids 409 and 750 of KDM6A (Uniprot ID#O15550) |
| Physical form: | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 - H412 |
| Precautionary statements | P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |