Anti-EV71 antibody produced in rabbit
SIGMA/SAB2702131
Synonym: EV71 inactive virus; Enterovirus 71
Product Type: Product-on-demand
Immunohistochemistry EV71 antibody detects EV71 viral protein in EV71 infected mouse skeletal muscle by immunohistochemical analysis. Sample: Paraffin-embedded EV71 infected mouse skeletal muscle. EV71 antibody [SAB2702131] diluted at 1:500
Immunofluorescence Immunofluorescence analysis of paraformaldehyde-fixed EV71 infected Vero cell, using EV71 (SAB2702131) antibody at 1:1000 and 1:5000 dilution.
Western Blotting Sample (25 μg of whole cell lysate) A: Vero cell lysate B: Vero cell lysate infected with EV-71 10% SDS PAGE SAB2702131 diluted at 1:20,000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blotting EV71 antibody detects EV71 protein by western blot analysis. Un-infected (-) and infected with EV71 C2 or B4 strain RD whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with EV71 antibody (SAB2702131) at a dilution of 1:20,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 6.15 mg/mL |
| conjugate | unconjugated |
| form | liquid |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | EV71 |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: suitable |
| immunocytochemistry: suitable | |
| immunofluorescence: suitable | |
| western blot: 5000-20000 |
| Application: | Suggested starting dilutions are as follows: ELISA: Assay-dependent dilution, ICC/IF: 1:100-1:1000, IHC-P: 1:100-1:1000, WB: 1:5000-1:20000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Enterovirus 71 |
| Physical form: | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 - H412 |
| Precautionary statements | P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |