Anti-HIF1A antibody produced in rabbit
SIGMA/SAB2702132
Synonym: HIF1-ALPHA; MOP1; PASD8; bHLHe78
Product Type: Product-on-demand
Immunohistochemistry HIF1 alpha antibody detects HIF1 alpha protein at nucleus on human kidney cancer by immunohistochemical analysis. Sample: Paraffin-embedded human kidney cancer. HIF1 alpha antibody (SAB2702132) dilution: 1:500.
Immunofluorescence HIF1 alpha antibody detects HIF1 alpha protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HIF1 alpha protein stained by HIF1 alpha antibody (SAB2702132) diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar = 10 μm.
Immunofluorescence HIF1 alpha antibody detects HIF1 alpha protein at nucleus in CoCl2-treated samples by confocal immunofluorescent analysis. Samples: Untreated (left panel) and 500μM CoCl2 treated (right panel) HeLa cells for 24 hrs were fixed in 4% paraformaldehyde for 15 min Green: HIF1alpha protein stained by HIF1 alpha antibody (SAB2702132) diluted at 1:1000 Red: Alpha-tubulin, a cytoskeleton marker, stained by Alpha-tubulin antibody diluted Blue: Hoechst 33342 staining. Scale bar = 10 μm. Images captured by Olympus FV10i Confocal Laser Scanning Microscope
Immunofluorescence HIF1 alpha antibody detects HIF1 alpha protein at nucleus by immunofluorescent analysis. Sample: NIH/3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HIF1 alpha protein stained by HIF1 alpha antibody (SAB2702132) diluted at 1:200. Blue: Hoechst 33342 staining.
Western Blotting HIF1 alpha antibody detects HIF1 alpha protein by western blot analysis. Un-treated (-) and treated (+, 1% O2 treatment for 24hr) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (SAB2702132) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blotting Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (SAB2702132) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blotting Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (SAB2702132) diluted at 1:1000.
Western Blotting Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (SAB2702132) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blotting Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (SAB2702132) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blotting HIF1 alpha antibody detects HIF1 alpha protein by western blot analysis. A. 30 μg HepG2 whole cell lysate/extract (untreated) B.30 μg HepG2 whole cell lysate/extract ( 200 μM CoCl2 treatment for 24 hr) C. 30 μg HepG2 whole cell lysate/extract (500 μM CoCl2 treatment for 24 hr) 7.5% SDS-PAGE HIF1 alpha antibody (SAB2702132) dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunoprecipitation Immunoprecipitation of HIF1 alpha protein from HepG2 whole cell extracts treated with 500 μM CoCl2 for 24 hr using 5 μg of HIF1 alpha antibody (SAB2702132). Western blot analysis was performed using HIF1 alpha antibody (SAB2702132). EasyBlot anti-Rabbit IgG was used as a secondary reagent.
ChIP Assay ChIP was performed with HepG2 chromatin extract and 5 μg of either normal rabbit IgG or anti-HIF1 alpha antibody. The precipitated DNA was detected by PCR with primer set targeting to VEGF promoter.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| form | liquid |
| mol wt | 93 kDa |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | mouse, bovine, human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ChIP: suitable |
| immunocytochemistry: suitable | |
| immunofluorescence: suitable | |
| immunohistochemistry: 1:100-1:1000 | |
| immunoprecipitation (IP): suitable | |
| western blot: 500-10000 | |
| UniProt accession no. | Q16665  |
| Application: | Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IHC-Fr: Assay-dependent dilution, IHC-P: 1:100-1:1000, IP: 1:100-1:570, WB: 1:500-1:10000, ChIP assay: Assay-dependent dilution. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Biochem/physiol Actions: | Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified. [provided by RefSeq] |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Recombinant fragment contain a sequence corresponding to a region within amino acids 657 and 826 of HIF1 alpha |
| Physical form: | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 - H412 |
| Precautionary statements | P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |