Monoclonal Anti-CEP164 antibody produced in mouse
SIGMA/SAB2702133
Synonym: Cep164 Antibody; Cep164 Antibody - Monoclonal Anti-CEP164 antibody produced in mouse; NPHP15
Product Type: Product-on-demand
Western Blotting Cep 164 antibody [13] detects Cep 164 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 5% SDS-PAGE, and blotted with Cep 164 antibody [13] (SAB2702133) diluted by 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Western Blotting Sample (30 μg of whole cell lysate) A: HeLa 5% SDS PAGE SAB2702133 diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 13 |
| monoclonal | |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| form | liquid |
| isotype | IgG1 |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunofluorescence: suitable | |
| immunoprecipitation (IP): suitable | |
| western blot: 1:500-1:3000 | |
| UniProt accession no. | Q9UPV0  |
| Application: | Monoclonal Anti-CEP164 antibody produced in mouse has been used in immunoblotting (5 μg/ml). |
| Biochem/physiol Actions: | The centrosomal protein 164 (CEP164) plays an important role in the synthesis of the primary cilium. This protein interacts with several proteins during ciliogenesis such as Rabin-8, Tau tubulin kinase‐2 (TTBK2), coiled-coil domain containing 41 (CCDC41), and dishevelled (DVL). CEP164 protein is also involved in G2/M checkpoint and nuclear divisions. This protein is a key player in the DNA damage-activated ataxia telangiectasia mutated (ATM)/Rad3-related (ATR) signaling cascade since it is required for the proper phosphorylation of the histone family member X (H2AX), replication protein A (RPA), checkpoint kinase 1 and 2 (CHK1/2). Mutations in the CEP164 gene leads to a condition known as nephronophthisis (NPHP)‐related ciliopathies. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
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| General description: | The centrosomal protein 164 (CEP164) is expressed at the distal ends of the mother centriole. This protein consists of an N-terminal WW domain and three coiled-coil regions. The CEP164 gene is located on the human chromosome at 11q23.3. |
| Immunogen: | Human Cep164 |
| Physical form: | Phosphate-buffered saline, no preservative added. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |