Monoclonal Anti-CENPF antibody produced in mouse
SIGMA/SAB2702140
Synonym: CENF; PRO1779; hcp-1
Product Type: Product-on-demand
Immunohistochemistry CENPF antibody [14C10 1D8] detects CENPF protein at nucleus on BT483 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded BT483 xenograft. CENPF antibody [14C10 1D8] (SAB2702140) dilution: 1:200.
Immunofluorescence Confocal immunofluorescence staining (Olympus FV10i) of Mitosin. U2OS cells were fixed by 4% PFA and costained with Mitosin 14C10 1D8 AB (Red; SAB2702140; 1:500 Ab dilution) and alpha-tubulin rabbit polyclonal AB (Green), a spindle marker. DAPI (blue), chromosomes. Scale bar, 5 um
Western Blotting CENPF antibody [14C10 1D8] detects CENPF protein by western blot analysis. A. 30 μg HeLa whole cell lysate/extract (untreated) B. 30 μg HeLa whole cell lysate/extract (100ng/ml Nocodazole treatment for 24hr) 5% SDS-PAGE CENPF antibody [14C10 1D8] (SAB2702140) dilution: 1:500 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 14C10 1D8 |
| monoclonal | |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| form | liquid |
| isotype | IgG2a |
| mol wt | 350 kDa |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: suitable |
| immunocytochemistry: suitable | |
| immunofluorescence: suitable | |
| immunohistochemistry: suitable | |
| immunoprecipitation (IP): suitable | |
| western blot: suitable | |
| UniProt accession no. | P49454  |
| Application: | For IHC-Fr: Use at an assay dependent dilution. For IHC-P: Use at an assay dependent dilution. For IP: Use at an assay dependent dilution. For WB: Use at a dilution of 1:500. Predicted molecular weight: 350 kDa. Optimal dilutions/concentrations should be determined by the researcher. |
| Biochem/physiol Actions: | Mitosin-14C10 recognizes mitosin, a 350kDa phosphoprotein that is specifically involved in mitotic-phase progression. Mitosin is expressed throughout S, G2, and M phases of the cell cycle but is absent in G0 and G1. It associates with the mitotic apparatus during M-phase, and is redistributed from the nucleus to the centromere, spindle, and midbody during M-phase progression. Its usefulness as a proliferation marker is under active investigation. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Protein fragment expressed in E. coli corresponding to amino acids 1759-2093. |
| Physical form: | Phosphate-buffered saline, no preservative added. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |