Monoclonal Anti-POLD1 antibody produced in mouse
SIGMA/SAB2702144
Synonym: CDC2; CDC2; POLD
Product Type: Product-on-demand
Western Blotting Detection of human DNA polymerase delta by using anti-pol delta monoclonal antibody [607] (SAB2702144) in HeLa whole cell extract. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Western Blotting Sample (30 μg of whole cell lysate) A: BCL-1 5% SDS PAGE SAB2702144 diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 607 |
| monoclonal | |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| form | liquid |
| isotype | IgG |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1:1000 |
| UniProt accession no. | P28340  |
| Application: | For WB: Use at a concentration of 1:1,000. Predicted molecular weight: 130 kDa. Optimal dilutions/concentrations should be determined by the reseracher. |
| Biochem/physiol Actions: | DNA polymerase d (delta) functions in DNA replication and DNA repair. It also possesses 3′ to 5′ exonuclease activity. In leading strand synthesis it requires the processivity factor, PCNA. It is also possibly involved in the completion of DNA synthesis during Okazaki fragment elongation following the initiation of synthesis by the DNA polymerase a/primase complex |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Recombinant protein encoding amino acids 1-140 of the large subunit of human DNA polymerase d. |
| Physical form: | Phosphate-buffered saline, pH 7.2, containing no preservatives |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |